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  • Author: Jiang-Yan Li x
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Tapered fiber sensor in the near infrared wavelength


Simulated transmission spectra for tapered fibers with no taper, one taper and two tapers in the near infrared wavelength range, calculated by Finite-Difference-Time-Domain method are currently presented. Transmission peak positions tend to shift to the shorter wavelength when the taper deformation is added to the fiber or the taper width gets narrower. The thickness sensitivity for the tapered structures with different taper thicknesses is about 2.28e-3 nm·μm−1. There is an interference structure in the electric field distribution images, which reveals in the fiber structures. The transmission spectra for the fiber without taper, one taper and two-tapered structures were simulated in near infrared wavelength by FDTD. The transmission spectra for tlated in near infrared wavelength by FDTD. The sensitivity of the fiber was about 50 nm × RIU−1 and it had better refractive index detection. The tapered fiber can be applied to the bio-chemical sensors and physical deformation testing.

Open access
Original Article. Evaluation of Rapid Detection of Nasopharyngeal Colonization with MRSA by Real-Time PCR


Objective To investigate the clinical application of Real-Time PCR for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from nasopharyngeal swab specimens.

Methods We collected the nasal and throat swab specimens from patients or medical staffs in 3 intensive care units, blood laminar flow ward and respiratory ward in Beijing Hospital, Ministry of Health from December 2010 to April 2011. Each sample was tested by RT-PCR and conventional culture-based method for the presence of MRSA.

Results The total number of the specimens was 206. Compared with the conventional culture-based method, we demonstrated the diagnostic values for Real-Time PCR were 96.4% sensitivity, 96.6% specificity, 81.8% positive predictive rate, and 99.4% negative predictive rate. And the limit of detection was 102CFU/ml.

Conclusions This Real-Time PCR is a simple, rapid, sensitive and specific method. With the high negative predictive value, it can be used for the exclusion of MRSA colonization or infection. However, the application of its low positive predictive value should be further evaluated.

Open access
H-type hypertension and risk of stroke in chinese adults: A prospective, nested case–control study


Objectives: To investigate the independent and joint associations of hyperhomocysteinemia and hypertension with incident stroke and stroke death in Chinese adults.

Methods: About 39,165 rural Chinese adults aged 35 years or older who had no history of stroke at the baseline study were prospectively followed to determine major cardiovascular events, with an average follow-up of 6.2 years. Using a nested case–control design, this report includes 179 incident stroke cases (121 stroke deaths) and 179 controls without vascular events from the original cohort matched by age, sex, community, and length of plasma storage. Baseline plasma total homocysteine (tHcy) measurements were obtained for all subjects. Logistic regression analysis was performed to investigate the independent and joint associations between H-type hypertension, defined as subjects with concomitant hypertension and elevated homocysteine (≥10 μmol/L), and risk of incident stroke and stroke death, after adjusting for important covariates.

Results: We analyzed each risk factor independently and jointly. For analysis, homocysteine was divided into three groups: low (tHcy <10 µmol/L), moderate (≥10 µmol/L tHcy <20 µmol/L), and high (tHcy≥20µmol/L). Compared to subjects in the low group, the odds ratios (95% CI) of incident stroke for those in the moderate group and the high group were 1.7 (0.8–3.7) and 3.1 (1.2–8.6), respectively. The odds ratios (95% CI) of stroke death for the moderate and high groups were 2.8 (1.1–7.4) and 5.1 (1.6–16.4), respectively. Hypertension was also independently associated with a higher risk of incident stroke and stroke death: 3.8 (2.3–6.4) and 3.2 (1.8–6.0), respectively, compared to those without hypertension. When analyzed jointly, the highest risk was found among patients with H-type hypertensive with both hyperhomocysteinemia and hypertension: 12.7 (2.8–58.0) for incident stroke and 11.7 (2.5–54.7) for stroke death.

Conclusions: This study provides strong evidence that hyperhomocysteinemia and hypertension are two independent, modifiable risk factors, which act additively to increase the risk of incident stroke and stroke death. The results strongly suggest that H-type hypertension is a major risk factor for vascular disease and mortality, and those with H-type hypertension may particularly benefit from homocysteine-lowering therapy along with anti-hypertension therapy in Chinese populations.

Open access
Identification of HIV-1 Genotypic Resistance in Patients on First-line Antiretroviral Therapy Using Polymerase Chain Reaction and Sequencing


Objective The aim of the study was to evaluate the characteristics of HIV drug-genotypic resistance among patients taking first-line ARV regimens using polymerase chain reaction and sequencing, and guide to design optimal ARV regimens for these patients.

Methods HIV reverse transcriptase-encoded gene was amplified with RT-PCR and amplified PCR products were aligned and comparatively analyzed with HIV resistance database to find drug-resistance mutations.

Results Twenty-eight PCR products were amplified and sequenced successfully in 30 serum samples of recruited HIV-infected patients with virologic failure. The resistance rate was 96%, mutations in NRT region were found in 26 patients (93%), while mutations in NNRT region were found in 27 patients (96%). M184V was the most common mutation (86%), K65R was selected in 14% of recruited individuals and TAMs occurred in 50% of patients, which resulted in resistance to NRTIs. Y181C and V179D were the most common mutations in NNRTIs and prevalence was 43% (12/28) and 36% (10/28), respectively, which resulted in cross-resistance to NNRTIs due to low-genetic barrier.

Conclusions Virologic failure may occur in long-term administration of first-line ARV regimens, and drugresistance mutations can be found in these patients, which resulted in resistance to first-line ARV regimens. We emphasized that HIV viral load assay and resistance assay were important tools to guide healthcare workers to design an optimal second-line ARV regimens for HAART-experienced individuals with virologic failure.

Open access