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Open access

Weiping Tao, Sheng Hu, Zhiwei Wang and Jian Fan

Abstract

Background: The genetic factors of colon cancer play an important role in the tumor development and growth. The incidence of colon cancers has greatly increased in China. However, few data is available for the relationship between human muth homolog 1 (hMLH1) gene mutation at site 415 and sporadic colon cancers in Chinese population. Objective: Investigate the relationship between G→C mutation in hMLH1 gene at site 415 and sporadic colon cancers in Chinese Han population. Methods: Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing techniques, the genotype of the hMLH1 gene was analyzed at site 415 in 97 cases of sporadic colon cancer patients and 138 controls. Reverse-transcription (RT)-PCR was used to determine the level of hMLH1 mRNA expression in normal colonic mucosa of patients with different genotype. Results: The frequency of genotype C/C at the 415 site of the hMLH1 gene was significantly higher in colon cancer patients than in controls. The expression levels of hMLH1 mRNA in normal colonic mucosa were similar in colon cancer patients with different genotypes. Conclusion: G’!C mutation in hMLH1 gene at site 415 may represent a genetic factor that is associated with sporadic colon cancer in a small group of Chinese Han population.

Open access

Jian Fan, Li Zhang, Qi long Wang and Hui Lin

Abstract

Background: Metastasis is responsible for most cancer-related death, and the metastatic spread of neoplastic cells may be related to the ability of migration and invasion. Chemokine receptor 9 (CCR9) plays an important role in cutaneous melanoma and prostate cancer cells migration and invasion.

Objective: Investigate the specific role of the chemokine-ligand (CCR9-CCL25) axis in the development of nonsmall cell lung cancer (NSCLC) metastasis.

Methods: Semi-quantitative reverse transcriptase-PCR, western-blot, flow cytometry, migration, and invasion assays were used to examine the function of CCR9 in the NSCLC cells.

Results: CCR9 was highly expressed in NSCLC patient cancer tissue. In addition, in vitro migration and invasion studies on human bronchial epithelial cells of the BEAS-2B and human squamous lung cancer cell lines NCI-H157 showed that migration in response to the CCL25 was inhibited by CCR9 antibody.

Conclusion: CCR9 might play an important role in the migration and invasion of the NSCLC cells.

Open access

Dansheng Lei, Guanhui Zhang, Jian Fan, Jing Yu and Shaoqing Hu

Abstract

Background: Ficolins are lectins that have been demonstrated to play an important role in innate immune response in a variety of diseases. Mycobacterium tuberculosis (M.tb) infection can trigger a series of changes in the host. However, the role of ficolin in tuberculosis is still unclear.

Objective: We investigated the expression of ficolin in peripheral blood mononuclear cell (PBMC) in TB patients and healthy control.

Methods: Using semi-quantitative RT-PCR, western blotting, and flow cytometry, we compared the expression of M-ficolin, L-ficolin, and H-ficolin in the peripheral blood mononuclear cell (PBMC), purified monocytes, and cultured dendritic cells of TB patients with healthy volunteers as controls.

Results: M-ficolin expression in PBMC was significantly lower at both mRNA and protein levels in TB patients as compared to healthy controls. The lower M-ficolin level in TB patient PBMCs may be attributed to its lower level in monocytes. The expression levels of H-ficolin and L-ficolin in both healthy controls and TB patients were very low and they had no significant differences between the two groups.

Conclusions: Compared to healthy controls, M-ficolin expression is significantly lower in TB patients. Measurement of M-ficolin may be a potential auxiliary tool to diagnose TB infection.

Open access

Shu-Hua Zhao, Fan Zhao, Jing-Ying Zheng, Li-Fang Gao, Xue-Jian Zhao and Man-Hua Cui

Knockdown of stat3 expression by RNAi inhibits in vitro growth of human ovarian cancer

Background. The aim of the study was to investigate the suppressive effects of pSilencer2.1-U6-siRNA-stat3 recombinant plasmids on the growth of ovarian cancer in vitro.

Material and methods. Three pairs of DNA template (stat3-1, stat3-2, stat3-3) specific for different target sites on stat3 mRNA were synthesized to reconstruct pSilencer2.1-U6-siRNA-stat3s, which were transfected into SKOV3 cells. The expressions of STAT3, BcL-2, cyclin D1 and C-myc in these cells were detected by Western blot and Northern blot. The cell cycle and the growth were determined by flow cytometry (FCM) and MTT assay, respectively. Cell apoptosis was determined by TUNEL staining.

Results. Of the three siRNAs, only siRNA targeting stat3-3 markedly suppressed the protein expression of stat3 in SKOV3 cells; MTT assay and FCM showed that transfection of stat3-3 siRNA could significantly suppress the growth of SKOV3 cells and arrest the cell cycle in vitro. TUNEL staining also showed massive apoptosis in SKOV3 cells transfected with stat3-3 siRNA.

Conclusions. pSilencer2.1-U6-siRNA-stat3-3 can significantly inhibit the STAT3 expression in human ovarian cancer cells resulting in the inhibition of the cancer growth and the increase of apoptosis of cancer cells.

Open access

Jian-Qiang Gao, Li-Ya Fan, Li Li and Li-Zhong Xu

Abstract

A novel method for feature extraction and recognition called Kernel Fuzzy Discriminant Analysis (KFDA) is proposed in this paper to deal with recognition problems, e.g., for images. The KFDA method is obtained by combining the advantages of fuzzy methods and a kernel trick. Based on the orthogonal-triangular decomposition of a matrix and Singular Value Decomposition (SVD), two different variants, KFDA/QR and KFDA/SVD, of KFDA are obtained. In the proposed method, the membership degree is incorporated into the definition of between-class and within-class scatter matrices to get fuzzy between-class and within-class scatter matrices. The membership degree is obtained by combining the measures of features of samples data. In addition, the effects of employing different measures is investigated from a pure mathematical point of view, and the t-test statistical method is used for comparing the robustness of the learning algorithm. Experimental results on ORL and FERET face databases show that KFDA/QR and KFDA/SVD are more effective and feasible than Fuzzy Discriminant Analysis (FDA) and Kernel Discriminant Analysis (KDA) in terms of the mean correct recognition rate.

Open access

Wen Xie, Hong Zhao, Yu Chen, Qin Zhang, Wei Lu, Wei Liu, Ai-rong Hu, Han-wei Li, Ping Feng, Ming-sheng Chen, Cun-jin Mei, Xiao-lin Guo, Xiao-hu Zhao, Jiang-bin Wang, Zheng-qin Fan, Jian-he Gan, Qing Xie and Jun Cheng

Abstract

Obejective Ademetionine 1,4-butanedisulfonate [S-adenosyl-L-methionine (SAMe)/Transmetil®, Abbott] has been available in China for more than 15 years, and it has been shown to reduce serum bilirubin and transaminase levels in viral hepatitis (VH) patients. However, no large-scale studies have focused on the impact of SAMe treatment regimen on reducing the serum total bilirubin (TBil) in VH patients with intrahepatic cholestasis (IHC). The primary purpose of this study was to evaluate the effectiveness of intravenous SAMe (Transmetil®) treatment in reducing the serum TBil by 50%.

Methods This retrospective, multi-center, cross-sectional medical record review involved patients aged ≥18 years. Records of 1 280 hospitalized VH patients at 16 sites diagnosed with IHC who had received intravenous SAMe 1 000 mg or 2 000 mg q.d. for at least 7 days from January 1, 2006 to June 30, 2009, were screened and 905 records were randomly selected.

Results The safety set (SS) included 834 patients and the full analysis set 826 patients. TBil levels after 14 days injection treatment were available for 763 patients. TBil decreased ≥ 50% versus baseline after 14 days treatment in 288 (37.7%) patients (95% CI 34.3%, 41.2%). Twenty-nine non-serious adverse events (non-SAEs) were reported in 19 (2.3%) patients, and 29 SAEs were reported in 10 patients (1.2%). All adverse events (AEs) were considered unrelated to the study drug.

Conclusions This retrospective study shows that intravenous SAMe administration in VH patients with IHC is associated with significant reduction of TBil levels in more than 30% of patients 14 days after treatment initiation.