Among the 2750 species of the Polish vascular flora, about 500 species are threatened with extinction and 430 of them are strictly protected by national law. The FlorNatur project for the ex situ conservation of the most endangered species was started in 2009. The aim of the project is to collect seeds of 61 species from 161 sites in eastern Poland and store them in the Seed Bank of the Polish Academy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Warsaw- Powsin. A complementary program is being carried out by the Forestry Gene Bank at Kostrzyca in western Poland. Their task is to collect 58 species from 129 natural sites in the western part of Poland. To date, seeds of 31 species from 56 populations have been collected, tested and stored in liquid nitrogen.
The study was performed on nasal swabs, tracheal samples, and sera obtained from young beef heifers aged between 6 and 12 months, from farms in eastern and south-eastern Poland. The samples were evaluated using bovine herpesvirus 1 (BHV-1) ELISA kits (ELISA BHV1 antibody and ELISA BHV1 antigen) and PCR. Among all the animals examined, 37 (32.2%) were positive in the ELISA BHV1 antigen test. The presence of BHV-1 was confirmed by PCR in 42 (36.5%) animals. In the ELISA BHV1 antibody test, 39 (33.9%) seropositive animals were identified. The presence of BHV-1 positive samples was observed in all the examined breeds of young cattle. There were no significant differences (P ≤ 0.05) in BHV-1 positive samples. The results indicate that the incidence of BHV-1 infections in feedlot cattle herds studied was 32.2%-36.5%, which suggests that preventive measures should be implemented in order to limit transmission of the virus.