Histochemical methods for the detection and diagnosis of the developmental stages of the canine tapeworm, from the genus Taenia found in the heart and lungs of red deer (Cervus elaphus) and roe deer (Capreolus capreolus) hunted in Eastern Slovakia, is presented here. Detailed morphology of cysticerci (Cysticercus spp.), based on microscopic and histochemical analysis is described. For confirmation and demonstration of PAS-positive substances in the body of parasitic tissue (tegument and mesenchyme) the McManus — PAS method was used. The histochemical method according to Van Kossa was very effective for confirmation of calcareous corpuscles, which are one of the most important histological markers of cestode tissues (larva or adult).
The role of selenium has been changed over the last decade. The element that was previously considered to be toxic turned out to be present in the human body in amounts of 10–15 mg, and almost every cell of our body contains it. Selenium contributes to growth, supports healthy muscle activity, reproductive organs, reduces the toxicity of certain elements such as mercury, supports the immune system, and even delays the spread of certain viruses (influenza, Ebola, HIV). Selenium-deficient areas of Europe could be a risk for their populations. The recommended daily intake (RDA) of selenium is 55 µg/day, while WHO and FAO have set up the daily tolerable dose at 400 µg/day. We must count with the harmful effects of selenium overdose, but it is almost impossible to introduce this amount into our body solely with food. Our selenium sources can be refilled with food supplements or selenium-enriched functional foods. In the review article, we report about the role of selenium in the environment, selenium-enriched plants, selenium-enriched yeast, the role of selenium in animal feed and in the human body, the opportunities of selenium restoration, selenium-enriched animal products, and the selenium content of milk.
Nowadays, number of customer and environmental requirements are laid to the automotive industry. These are related to safety increase, the weight and emission reduction as well as the life-time improvement. The article presents the way to improve the corrosion resistance of the car-body components when coated steel sheets are used based on Zn-Al-Mg coatings. Thus, the life-time of the car-body is improved. Samples with conventional Zn coating and samples with advanced Zn-Al-Mg coatings were deformed by stretching and 3-point bending to 90° and 180° tests. Consequently, the samples were exposed to salt spray in the corrosion chamber. Time to red corrosion appearance and adequate percentage of corroded surface was evaluated. The results showed the better corrosion protection of Zn-Al-Mg coatings when compared to the conventional Zn coating.
Syrian oregano (Origanum syriacum L., Lamiaceae) is a very popular culinary herb that has been used through the ages in traditional medicine mainly in Lebanon and the Arab world. The subject of this study was the microscopic analysis of the herbal drug Origani syriaci folium, as well as the chemical analysis of its essential oil components. Plant materials originated from Arabsalim - South Lebanon and were collected in 2010. The presence of very dense clusters of multicellular covering trichomes (510 μm × 18.47 μm) and peltate secretory glands (∅ 87.95 μm) with eight cells were observed. The essential oil of Origani syriaci folium was isolated and quantified using hydrodistillation according to SPhC 1997. The content of essential oil was 95 ml/1000 g, i.e. 9.5% (V/m). The volatile constituents of Origani syriaci folium were qualitatively and quantitatively evaluated using SPME GC/MS. The two dominant constituents identified in Syrian oregano leaves were carvacrol (78.4%) and thymol (17.9%).
Salvia officinalis L. (sage) is an important essential oil containing Mediterranean medicinal plant that is commonly cultivated for pharmaceutical uses. In addition to essential oil, other compounds participate on the biological effects of sage, mainly diterpenes, triterpenes and phenolic compounds of the hydroxycinnamic and flavonoid types. Sage essential oil content is known to differ in plant parts, and to be influenced by plant phenophase, climatic and edaphic conditions. This work deals with the study of total hydroxycinnamic derivatives (THD), and specifically rosmarinic acid (RA), content variation in different aerial parts of sage linked to different harvest times. Dry sage leaves (Salviae officinalis folium) THD content was quantified using a pharmacopoeial method, and varied between 3.06 % and 3.52 % in different harvest times, with maxima in youngest plant shoot leaves and newly-grown shoots in September. Sage stems showed similar THD content variations when linked to harvest times, however with lower percentage, 1.33 - 3.04 %. Rosmarinic acid variability showed the same trends, its content ranged between 0.76 % and 1.65 % in leaves, and between 0.19 % and 0.83 % in stems, respectively. Highest percentages of both THD and RA were found in top leaves, lowest in leaves from the middle of the stem. When linked to plant phenophase, the content of THD and RA decreased at flowering time.