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Open access

I. Dolka, A. Giżejewska, Z. Giżejewski, J. Kołodziejska-Lesisz and W. Kluciński

Abstract

Adiaspiromycosis is a rare fungal infection caused by saprophytic fungi Emmonsia spp. (type Ascomycota) occurring especially in small free-living mammals. The aim of this study was to evaluate the occurrence of histopathological lesions asscociated with adiaspiromycosis in the Eurasian beaver inhabiting Poland. In order to evaluate the presence of natural adiaspiromycosis we systematically investigated beaver populations from north-eastern Poland for adiaspores in the lungs. This study reveals for the first time the presence of pulmonary adiaspiromycosis of Eurasian beaver in Poland. As far as we know, there is no published data regarding pulmonary adiaspiromycosis in human patients in Poland.

Open access

I. Dolka, R. Sapierzyński, W. Bielecki, E. Malicka, A. Żbikowski and P. Szeleszczuk

Abstract

The aim of this study was to estimate the prevalence of histopathological lesions in the different organs in relation to the commercial-type and the age of birds (i.e. broiler chickens and layers). During the period 1999-2010 a total of 189 cases was submitted to the Division of Animal Pathomorphology, Department of Pathology and Veterinary Diagnostics at WULS. Most cases were found in broiler chickens (66.7%). The majority of the histopathological lesions were detected in the liver and lymphoid organs. In of 29% cases of hepatic injury pathognomonic lesions associated with inclusion body hepatitis (IBH) were found. The mean age of birds was 23 days. Among IBH cases proventriculitis (58%) was more often found than gizzard lesions (25.8%). Interestingly, we noted some intranuclear inclusions in the epithelial cells within the proventriculus. A low percentage of histopathological evidence of infectious bursal disease (IBD) was reported in chickens. The gastrointestinal tract was the second most frequent predilection site for histopathological lesions. Histopathological findings within the heart and lungs were less common and were more often seen in the upper respiratory tract. Cases of infectious laryngotracheitis (ILT) were registered in broiler chickens (3.2%, mean age 37 days) and in layers (4.8%; mean age 196 days). Lesions associated with Marek’s disease, avian leukosis and fowl pox were recognized only in layers, respectively in 3.2% (mean age 176 days), 1.6% (mean age 205 days) and 1.1% (mean age 196 days) of all cases. Avian encephalomyelitis (AE) was noted only in 0.5% of all cases.

Open access

Barbara Osińska, Aleksander W. Demiaszkiewicz, Anna M. Pyziel and Izabella Dolka

Abstract

The aim of the study was to determine the prevalence of canine dirofilariosis in four provinces of central-eastern Poland and to describe pathological changes occurring during the course of this parasitosis. A total of 188 dogs aged from one and a half up to 16 years were examined for dirofilariosis. Blood samples were analysed for the presence of microfilariae using Knott method, as well as the method of Kingston and Morton. Pathomorphological examination of two dogs was also performed. Histological sections were stained with H&E, AB-PAS, van Gieson, and von Kossa methods. Microfilariae of Dirofilaria repens were found in blood samples of dogs that originated from the Mazowieckie, Lubelskie, and Podlaskie provinces. The mean prevalence of that species was 12.7%. Autopsy revealed the adult nematodes in the subcutaneous connective tissue. Microscopic examinations indicated the presence of microfilariae in the liver, kidneys, lungs, heart muscle, and intestines. Necrotic foci, thrombosis, and infiltrations of different intensity with eosinophils in internal organs were also observed. Significant lesions in different internal organs together with the presence of numerous microfilariae suggest their important role in the disease process.

Open access

Monika Olszewska-Tomczyk, Izabella Dolka, Edyta Świętoń and Krzysztof Śmietanka

Abstract

Introduction

Genotype VI of avian avulavirus 1 (AAvV-1) has pigeons and doves as its reservoir and is often termed pigeon paramyxovirus type-1 (PPMV-1). The pathogenesis of PPMV-1 infections in poultry is largely obscure. It is known that PPMV-1 requires a series of passages in chickens before it becomes adapted to gallinaceous poultry.

Material and Methods

Changes in the genome of PPMV-1 were analysed after serial passages in specific pathogen free (SPF) chickens, using high-throughput sequencing. Additionally, histopathological lesions induced by PPMV-1 in experimentally inoculated pigeons, chickens, and turkeys were evaluated.

Results

Following six passages of PPMV-1 in chickens, 10 nonsynonymous substitutions were found including one (in the NP protein) which dominated the genetic pool of viral quasispecies. Histopathological changes induced by the post-passage PPMV-1 strain were more prominent than changes wrought by the pre-passaged PPMV-1 strain and the lesions were most intense in pigeons followed by chickens and turkeys.

Conclusion

PPMV-1 is highly adapted to pigeons and passaging through chickens results in the acquisition of novel amino acids in the polymerase complex, which may alter the pathogenic potential of the virus.

Open access

Anna Kycko, Agnieszka Jasik, Łukasz Bocian, Iwona Otrocka-Domagała, Mateusz Mikiewicz, Anna Śmiech, Wojciech Łopuszyński, Izabella Dolka, Marcin Nowak and Janusz A. Madej

Abstract

Introduction: Apocrine sweat gland carcinomas (ASGCs) are malignant neoplasms of dogs and other animals, rarely reported worldwide. The aim of this study was to summarise the occurrence of this cancer in a population of dogs in Poland between 2009 and 2014 with regards to histological features and body location of the tumours, as well as age, sex and breed of the cancer-affected dogs.

Material and Methods: The study involved 40 canine ASGC cases diagnosed in five national veterinary pathology laboratories. The material was processed according to routine histological methods.

Results: Histological types of the tumours involved simple and complex apocrine carcinoma of cystic/papillary (62.5%), solid (15%), and tubular type (12.5%), as well as apocrine ductal carcinoma (10%). The epidemiological analysis revealed peak incidence of the cancer in dogs between 8 and 14 years of age, with the most commonly affected sites being forelimbs and thorax. The highest number of the cancer cases was diagnosed in mixed breed dogs and German Shepherds; no sex predilection was noted.

Conclusion: To the authors’ knowledge, this is the first report recounting the study on canine malignant apocrine sweat gland tumours in Poland providing detailed phenotypical and histological data, which are otherwise rarely described in veterinary literature. This type of cancer appears to be diagnosed more frequently in dogs than in humans. Being an easily accessible material for research, canine ASGCs might serve as a relevant animal model for studies related to pathogenesis of sweat gland tumours.