Macrofauna plays a very important role in the functioning of the natural environment. It plays an important role in the decomposition of organic matter by mixing and crushing organic matter in soil. Invertebrate faeces influence the development of microorganisms and their dead bodies stimulate mineralization in the soil. They also influence the humification processes. The aim of the study was to determine the influence of macrofauna and litter distribution and the accumulation of organic carbon in soil. The study showed a significant influence of this thick animal on the processes taking place in the soil. Significant correlations were observed between the organic carbon content in the litter and the organic carbon content in the soil, macrofauna activity with litter decomposition and its influence on the organic carbon accumulation.