Segment videos were produced at different peaks to reflect different sampling criteria like land use characteristics, trails, Ciclocarrils and Ciclovia. Each segment was filmed for 20–40 seconds during bicycle rides at a speed of about 5km/h with a camera strapped, at an angle of 45 degrees, on the head. Curb lane variables such as bicycle pathway widths, curb lane motorised volume (veh/h) and vehicle speed (km/h), bicycle volume on segment, and median width were recorded in addition to secondary data. About 1,360 ratings were acquired from study participants and used in the estimation process. Ordered probability models were used to estimate random parameters of cyclists LOS perception to account for unobserved heterogeneity for all respondents. The deviance (1.085) and Pearson Chi-Square (2.309) with 1,635 degree of freedom at 0.05 level of significance shows that our model provides a better fit of the data. The study observed that BLOS was strongly influenced by side path separation, vehicle speed, motorised traffic volume and conflicts with pedestrians. However, many other factors were found to have high probabilities to influence level of service with unit change. They include bicycle lane width, wide outside lane, pavement conditions, trees and benches, daylight, gender and experience of cyclist. The impact of the variety of observed factors affecting bicyclists reveal the nature and character of urban transportation in Bogota which suggests a range of important trade-offs in further planning and management of the Cicloruta bicycle paths.