The purposes of the current research were to deposit the silver nanoparticles on the surface of a textile woven fabric and evaluate their dyeing performance and antibacterial activity. The synthesis of silver nanoparticle (Ag°) is done by the in situ method. Strong alkali is used to improve functionality of cellulose before the application of silver nitrate salt (AgNO3). The silver nanoparticle is formed by reduction of ascorbic acid. Various instrumental analyses are done to prove the formation of nanoparticles on the fabric surface. The morphology of nanodeposited fabric is characterized by using scanning electron microscope (SEM), elemental composition is done by energy dispersive spectroscopy, and crystallinity of nanoparticles is obtained by X-ray diffraction (XRD). Nanodeposited fabric is then dyed with direct dyestuff (Direct Red-89). Fourier transform infrared spectroscopy analysis is done to explore the bonding phenomena of un-dyed and dyed fabrics. The dyeing performance and antibacterial activity are examined on the colored fabric to investigate the dyed fabric quality after nanoparticle deposition. Results demonstrate the improvement of 54% of color strength and 11% of dye exhaustion with excellent antibacterial activity.