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  • Author: Ibolya Fülöp x
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Objective: Alcohol use can cause social problems. Beside alcoholism the “binge drinking” and the “pre-drinking” could be a harmful form of alcohol use based on scientific literature data. In this study the alcohol use behaviors and associated problems were evaluated among young people aged between 14-30 years.

Methods: The study was carried out using a self reporting questionnaire, containing the Alcohol Use Disorders Identification Test (AUDIT) developed by World Health Organization and 10 more questions. This questionnaire was created in two languages (Romanian, Hungarian) and uploaded to the on-line survey page ( The link was shared at different Facebook groups.

Results: The questionnaire was completed by 933 young people. There was a predominance of females (66.99%) and the participant’s average age was 22.12 years (±2.78). The results show that males’ relative risk (RR) for alcoholism is 7.18 (CI95%, 3.47-14.85). Majority (539; 57.77%) of the participants had at least one time binge drinking, from this 36.66% were occasionally binge drinkers and 21.11% were regular binge drinkers. More males binge drinkers were found then females (RR: 1.72; CI95%, 1.55-1.90). The people who participate in pre-drinking have RR of 2.14 (CI95%, 1.89-2.41) for binge drinking. The binge drinkers have RR of 2.58 (CI95%, 1.78-3.37) for drunk driving.

Conclusions: Binge drinking is a really common habit among young people. Measures to reduce the risk or prevent the harmful consequences of this habit should be employed.


A method of measuring in vivo nitric oxide (NO) levels is required to detect pathological conditions in which endogenous production is decreased or to identify agents able to release this biomolecule. Unfortunately, nitric oxide has a very short biological half-life and is very difficult to measure. Assay of the oxidative products’ of NO levels, nitrite (NO2 -) and nitrate (NO3 -), measured as total amount, after the reduction of nitrate to nitrite, determined after conversion in an azo dye, is usually the used method, named NOx test. The NOx test is frequently used as a NO biomarker in human studies and also in animal experiments. The aim of this work is to evaluate the suitability of the NOx test for the detection of an instant release of nitric oxide.

Rabbits were used as experimental animals, a validated HPLC-UV/VIS method was used for speciation of nitrite and nitrate. The following substances were administered: blank; “negative blank”: phenyl-N-tert-butylnitrone (PBN); “positive blank” (nitroglycerin); nitrite.

PBN administration significantly increased nitrate and decreased nitrite levels, nitrite administration excessively increased nitrate levels, while nitroglycerin (1 mg/kg) significantly increased both nitrate and nitrite levels.

Results show that NOx test cannot be considered accurate in acute nitric oxide status testing. Nitrite alone should be used as an in vivo released nitric oxide marker.


Objective: One of the most important sources of nitrite and nitrate anions, besides vegetables and meat products, is the drinking water. Presence of nitrite and nitrate in the water in higher concentrations than those set by EFSA (0.5 mg/l nitrite, 50 mg/l nitrate), may have toxicological significance. A quantitative determination of these ions in samples collected from several pleases from Mureș County was made.

Methods: Ninety-seven well water samples were tested from 12 different places from Mureș County. We used a simple HPLC-UV ion pair method for the determination of nitrite and nitrate concentrations. Sensitivity of the method enables the quantification for concentrations far below the MCL value.

Results: The highest amounts of nitrate and nitrite were measured in Sangeorgiu de Mureș and Cristești. Concentrations of nitrite and nitrate were exceeded in 4.12% and respectively 44.32% of the samples.

Conclusions: The high amounts of nitrites and nitrates existing in well water go beyond the expected extent. This pollution can become a health risk since this water is used in human nutrition especially in child nourishment.


Objective: Methanol appears in relatively high concentrations in alcoholic beverages obtained from fermented fruits distillates. These products are traditionally home made in many regions in Romania and other EU countries. The chronic use of products with high methanol concentration can be considered a health risk. The purpose of this work was to measure methanol concentration in a Romanian region where brandy-type alcoholic products are made from different fruits (plum, apple, pear, grapes), and to observe if there is a type of product that contains more methanol than the others. Methods: The content of methanol in the tested alcoholic beverages was determined using a gas chromatographic method. Results: Only 18% of the tested 56 samples met UE regulation regarding methanol content of alcoholic beverages (0.4% in alcoholic drinks containing 40% ethanol). The highest concentration of 2.39% was found in a plum brandy. Plum brandies contained significantly higher amounts of methanol than brandies made from other fruits (0.91 vs 0.52%, p = 0.01). Conclusions: Home distilled alcoholic beverages obtained from fruits are a health threat due to their high methanol content. Strict regulations and tests should be introduced for such products


Objective: the number of alkaloids like morphine and codeine found in poppy seeds used in food industry are monitored by a directive given by European Food Safety Authority. Based on this regulation the aim of the study was to determine the quantity of morphine and codeine from several brands of poppy seeds. Methods: an HPLC-UV method (205 nm) was developed to measure the quantity of morphine and codeine. Sample preparation was made using recipes posted on Drugs Forum by some users. Limits of detection were not determined because the lowest concentration from the reference (0.1 μg/ml) detected morphine concentrations that are far lower than a limit of toxicological concern. Results: The concentrations, which were found, ranged between Below the Level of Toxicological Concern (BLTC) - 243.26 mg/kg for morphine and BLTC - 88.58 mg/kg for codeine using several methods of preparation. Conclusions: one can observe that there are some brands of poppy seeds which do not respect the regulation about the amount of morphine and codeine. The high amount of morphine in some samples suggests that there are different varieties of poppy seeds, which can be used for an illicit purpose and can lead to addiction or even overdose in some cases.


Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as biomarkers of nitric oxide production. Most nitrones did not increase plasma concentrations of nitrite and nitrate; on the contrary, reduced plasma concentrations of these indicators were noted. However, glyoxal isopropyldinitrone, in a dose of 50 mg kg-1, was highly effective in increasing nitric oxide production. At the same time, nitrones do not react with hepatic homogenates, proving that the release of nitric oxide takes place in the tissues and is not related to hepatic metabolism. Before using nitrones in vivo, they were tested in vitro for the ability to release nitric oxide following a reaction with the hydroxyl radical.


Offering a systematic and multivariate analysis of the analytical procedure, development and validation of HPLC methods using Quality by Design approach are in the limelight of current research trends. A new, experimental design-aided HPLC method for fampridine was developed and preliminarily validated according to current in-force international guidelines for linearity, accuracy, robustness and precision.

The method offers a high throughput sample analysis, with an elution time of 2.9 minutes, and signal detection without excipient interference performed at 262 nm. The method proved to be linear between 1–15 µg mL−1 (R2= 0.9996). The mean recovery was found to be 98.7 ± 1.9 % in the tested range of 2.5–7.5 µg mL−1. Low RSD values (< 1 %) were obtained for both model, intra- and inter-day precision. The limit of detection and limit of quantification were 0.24 and 0.78 µg mL−1, resp. The method proved to be applicable for active substance assay in a pharmaceutical dosage form.


Introduction: Camellia sinensis, a widely used plant, optimally grows in a low pH soil that in most cases contains high amounts of aluminum. Objectives: The aluminum content of the tea obtained from Camellia sinensis and other plants was compared. The influence of pH on the aluminum content of the tea was also measured. Materials and methods: The aluminum content of 48 samples was measured using a colorimetric method. The method is based on the ability of aluminum to form a stable complex with xylenol orange at low pH; this complex has an absorption maximum of 555 nm. Results: The method was validated for tea obtained with water and for tea obtained with water containing citric acid. The method proved linear over the rage of 0.7 – 7 ug/ml, coefficient of variation ranged between 2.6 – 7.68% (was dependent on the pH of the solution used to obtain the tea), accuracy was suitable for quantitative measurement (92.39-102.92%) and the complex proved to be stable for at least 1 hour. The following concentrations were measured: green tea (1.59 - 7.70 µg/ml), black tea (1.39 - 5.60 µg/ml), fruit tea (1.01 - 5.63 µg/ml) and herbal tea (1.03 - 5.24 µg/ml). Conclusion: The method proved useful and easily applicable for screening aluminum content of plants used for tea brewing. Camellia sinensis both green and black types had significantly higher aluminum contents than other type of teas. Adding citric acid, as would result from use of lemon juice, significantly increased the aluminum extraction from the plants used for tea brewing.


Objective: The aim of this study was to evaluate how the membrane transport of diclofenac on presence of different types of food is modified.

Methods: The interaction of diclofenac, as a pure substance and solid dispersions of the active ingredient, with different types of foods was investigated in vitro condition using a modified Franz diffusion cell.

Results: The amount of diclofenac transported through a lipophilic membrane was reduced by the presence of foodstuffs in artificial gastric juice and intestinal juice also, both in the case of the pure substance and solid dispersions also. The only exception was the case of milk in artificial gastric juice, when the amount of diclofenac in the receiving compartment increased about 2-fold compared to the fasted conditions simulating media. In the case of the solid dispersions of diclofenac the membrane transport increased in all cases compared to the pure substance, but was also reduced by the foods.

Conclusions: It was concluded that, the presence of different foodstuffs can influence the membrane transport of diclofenac – by inhibiting its solubility – and these differences observed in vitro can lead to modifications in the in vivo pharmacokinetic parameters. The lowest difference in diclofenac membrane transport was observed in the case of diclofenac:PEG 6000 solid dispersion prepared in 1:5 mass ratio.


Oxidative stress is an imbalance between free radicals or other reactive species and the antioxidant activity of the organism. Oxidative stress can induce several illnesses such as cardiovascular disease, neurodegenerative disorders, diabetes, cancer, Alzheimer and Parkinson. The biomarkers of oxidative stress are used to test oxidative injury of biomolecules. The indicators of lipid peroxidation (malondialdehyde, 4-hydroxy- 2-nonenal, 2-propenal, isoprostanes), of protein oxidation (carbonylated proteins, tyrosine derivatives), of oxidative damage of DNA, and other biomarkers (glutathione level, metallothioneins, myeloperoxidase activity) are the most used oxidative stress markers. Diseases caused by oxidative stress can be prevented with antioxidants. In human body are several enzymes with antioxidant capacity (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase) and spin traps. Antioxidants are synthetized in the organism (glutathione) or arrive in the body by nutrition (ascorbic acid, vitamin E, carotenoids, flavonoids, resveratrol, xanthones). Different therapeutic strategies to reduce oxidative stress with the use of synthetic molecules such as nitrone-based antioxidants (phenyl-α-tert-butyl-nitrone (PBN), 2,4-disulphophenyl- N-tert-butylnitrone (NXY-059), stilbazulenyl nitrone (STAZN), which scavenge a wide variety of free radical species, increase endogenous antioxidant levels and inhibits free radical generation are also tested in animal models.