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  • Author: Hongbin Wang x
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Open access

Shixia Zhang, Jiantao Zhang, Nan Zhang, Jiao Shi and Hongbin Wang

Abstract

The objective of this study was to describe laparoscopic-assisted colopexy (LAC) technique, and compare the extent of the surgical trauma after LAC and open colopexy (OC) by examing postoperative serum values of C-reactive protein (CRP) in dogs. Twelve healthy mixed-breed dogs with body weight ranging from 15 to 25 kg were used. Two portal sites were used for LAC procedures. OC was performed by laparotomy on linea alba. Colopexy was accomplished in all dogs without major intraoperative and postoperative complications. A permanent adhesion between the colon and abdominal wall was observed. There were statistically significant differences in serum CRP levels between LAC and OC immediately after the procedure and 1 d post operation. LAC had a similar pexy effect to OC, but had fewer surgical trauma than OC in dogs.

Open access

Jiasan Zheng, Shi Shu, Cheng Xia, Chuang Xu, Hongyou Zhang and Hongbin Wang

Abstract

Introduction: The differentially expressed proteins between healthy cows and those with footrot were identified to explore changes in protein profiles associated with the disease. Material and Methods: Out of 36 cows selected for the experiment, 18 footrot-affected cows were included in the treatment group (group T) and 18 unaffected cows were included in the control group (group C). Plasma samples from groups T and C were subjected to two-dimensional electrophoresis analysis and differentially expressed proteins were identified by matrix-assisted laser desorption/ionisation tandem time-of-flight mass spectrometry. Bioinformatics, including gene ontology analysis and pathway analysis, was used for analysing all proteins. Results: Out of 63 spots identified by 2DE, 33 were selected for mass spectrum analysis, which identified 11 differentially expressed proteins in 26 spots. Footrot led to changes in profiles in plasma proteins that were classified to the pathway of inflammatory response, complement, and blood coagulation, among others. Conclusion: This study provides evidence of the defence mechanisms of cows with footrot to explore strategies for treatment.

Open access

Jian-Tao Zhang, Jing-Tao Shao, Yun-Feng Liu and Hong-Bin Wang

Abstract

Ten mixed-breed female dogs were used in the study. Abdominal wall lifting was performed with a sterilised cotton strip. Four portal sites were used to complete gasless laparoscopic ovariohysterectomy (GLOHE) procedures. The proper and suspensor ligament, ovarian pedicle, and broad ligament of the uterus, uterine artery and vein were coagulated using bipolar electrocoagulation. After the uterine body was coagulated and cut, the end of the cervix was ligated with a loop suture. Blood samples were obtained before the surgery, immediately after the procedure, and on 1, 3, and 5 d postoperatively for the determination of interleukin-6 (IL-6) and C-reactive protein (CRP). No significant surgical complications occurred. After gasless laparoscopic ovariohysterectomy, a significantly higher serum IL-6 level was found immediately after the surgery and 1 d postoperatively when compared with the level observed after traditional laparoscopic ovariohysterectomy (LOHE). However, no significant differences were observed in CRP level between GLOHE and LOHE. GLOHE proved to be a safe and feasible procedure in bitches.

Open access

Ge Bai, Hui Li, Yansong Ge, Qianzhen Zhang, Jiantao Zhang, Mingzi Chen, Tao Liu and Hongbin Wang

Abstract

Introduction: The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury.

Material and Methods: Eighteen miniature pigs were randomly divided into three groups: a sham operated group (sham group, laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), and a hydrogen-rich saline intervention group (IRI + HRS group). Samples of hepatic tissue and serum were collected at the time of reperfusion and then 3 h, 1 d, and 3 d post reperfusion. Liver function, oxidative stress, autophagy-related mRNA genes, and protein expression were evaluated. Changes in cell and tissue ultrastructure were examined by transmission electron microscopy.

Results: Compared with the sham group, the level of autophagy of hepatocytes increased in the IRI and IRI + HRS groups, corresponding to high oxidative stress and severe liver function injury. Liver function, antioxidant content, autophagy levels, and liver injury were improved after intervention with HRS in the IRI + HRS group compared with the IRI group.

Conclusion: Intervention with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy.

Open access

Yin Bai-Shuang, Li Gao, Fu Lian-Jun, Fu Ying, Sha Wan-Li, Li Guo-Jiang and Wang Hong-Bin

Abstract

The aim of this study was to assess whether atipamezole can restrain telazol/xylazine induced expression of c-fos in the rat brain. Rats were injected with a mixture of 13.81 mg/kg telazol and 5.21 mg/kg xylazine, following 10 min later 0.522 mg/kg atipamezole. Thereon, the thalamencephal and cerebral cortex were removed one hour after the last injection. The level of Fos protein was measured in the brain tissue by Westernblot. The results revealed that atipamezole attenuates telazol/xylazine induction of c-fos expression in the thalamencephal and cerebral cortex. The results indicated that atipamezole is able to inhibit telazol/xylazine-induced c-fos expression in the rat brain, thus protecting it from nerve damage.

Open access

Jiawen Wu, Jianzhong Hu, Hongbin Lu, Qi Liao, Juanjuan Wang, Mingxin Wu, Chunhan Sun and Ming Kang

Abstract

Background: Spinal cord injury severely impairs fertility of young men. It can induce erectile dysfunction, ejaculatory dysfunction, and semen abnormalities. However, the precise mechanism is unclear.

Objective: We observed the effects of sacral nerve electrostimulation on sex dysfunction of male rats with spinal cord injury.

Methods: Thirty-six male Sprague-Dawley (SD) rats were randomly divided into three groups (n = 12): sham operated group, spinal cord injury model group, and electrostimulation group. The weight of genitals, related sex hormone index, and sperm motility were examined in each group after operation at two, four, and six weeks, respectively.

Results: Sacral nerve electrostimulation could improve testis and epididymis weight, regulate hormone secretion (including an increase in GnRH, tT, and ABP, but decrease in FSH and LH), and cause an increase in sperm count and motility.

Conclusion: Sacral nerve electrostimulation may be effective in treating sex dysfunction resulting from spinal cord injury.