Hanna Huliaieva, Iryna Tokovenko, Victor Maksin, Volodymyr Kaplunenko and Antonina Kalinichenko
The laboratory experiments have been found that soaking seeds Galega orientalis L. (Fodder galega) in nanoaquacitrates solutions of Mn (10 and 20 mg/dm3), Mo (4 mg/dm3) and Mg (2 and 4 mg/dm3), has been lead to germination energy rise, while Mn (10 and 20 mg/dm3) and Mo (4 mg/dm3) concentrations has been influenced germinating ability. At the same time, the soaking seeds in solution of nanoaquacitrates Mn (20 mg/dm3) had the biggest stimulatory effect on the accumulation 7 daily sprouts mass (on 18%). It has been shown that soaking seeds in nanoparticles Mn and Mo solutions leads to the increase of catalase activities (especially under the influence of manganese) and peroxidase activities (under molybdenum influence). Applying the method of chlorophyll a fluorescence in the field and greenhouse experiments with Galega orientalis L. plants, artificial infected with phytoplasma Acholeplasma laidlawii var. granulum st. 118 the following changes in the photosynthetic apparatus has been indicated: reduction in the length of the light-antenna, blocking transport of electrons in plastoquinone pool PSII with reducing the pool of electron acceptors. It has also been indicated that photochemical activity resistance of the photosynthetic apparatus decreases while its stability increases, as result of described above effects the concentration of chlorophyll a and b in plants leaves decreases. The above-mentioned negative effects have been deactivated through foliar treatment of infected Galega orientalis L. plants with nanoaquacitrates solution Mo (4 mg/dm3) that allow increasing of photochemical resistance of photosynthetic apparatus as well as chlorophyll content in leaves. The foliar treatment with Mn (20 mg/dm3) solution of the infected plants, in compared with infected plants without treatment, resulted in more significant increase of Ki value (which correlate to the ribulose-1,5-bisphosphate carboxylase/oxygenase activity), which is explaining anti-mycoplasma effect of this solution.