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  • Author: Hamed Bagheri x
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Abstract

Background

Atmospheric plasma jet has different medical applications due to its low temperature at room temperature. In recent years, the effect of nonthermal plasmas on cancer cells has been studied, and it has been shown that this type of plasma has anti-proliferative effects on cancer cells.

Objectives

To design a plasma jet handpiece, which can be used in cutting operations in less bleeding surgery, eliminating cancer cells without damage to healthy cells and reducing the duration of wound healing.

Methods

The plasma handpiece simply consists of a nozzle body and two cathode and anode electrodes and a fully insulated body against heat and high voltage. Argon is introduced into the handpiece, and by plasma treatment, it is used for special purposes. Each piece was made according to its own manufacturing process and by assembling; the final product of the atmospheric plasma jet handpiece was ready for testing. The jet pipeline was then tested, and the effective parameters were examined.

Results

The cold atmospheric plasma jet length depends on factors such as power supply, applied voltage, gas flow rate and the distance between the electrodes. The results showed with increasing velocity, the flame and jet lengths decreased greatly due to high losses of plasma, including ions and electrons. Also with increasing the velocity of argon gas, its concentration decreased.

Conclusions

It is concluded that the performance of the proposed design is successful. The advantages include low-cost manufacturing, highly stable performance, and low erosion and can be considered for future development.

Abstract

The present study evaluated the effects of dietary inulin on growth performance, body composition, serum, biochemical, and mucus immune factors; as well as innate immune responses of rainbow trout fry challenged with Aeromonas hydrophila. Four diets were prepared using a commercially available fish feed as a basal diet and different levels of prebiotic inulin incorporation; 0 (control), 1, 2, and 3%; referred to as C, T1, T2, and T3, respectively. The findings of the 60-day feeding trial showed that inulin inclusion affected final weight, food conversion rate (FCR), and specific growth rate (SGR) compared to that of the control group (P < 0.05), in which the lowest FCR was observed in T3. Body composition analysis revealed that inulin significantly increased protein content and decreased lipid levels, especially in the T1 and T2 groups. The lowest ash level was noticed in T2 (P < 0.05). Blood total protein, albumin, globulin, cholesterol, and glucose were not affected by inulin supplementation (P > 0.05). Analysis of humoral immune responses showed that the inulin supplements significantly increased lysozyme and complement activities (P < 0.05), as well as higher red blood cell count (RBC) and hemoglobin (Hb) in fish, fed 2% inulin, while no significant differences were observed among other treatments (P > 0.05). The mucosal parameters; including lysozyme, alkaline phosphatase (excluding ACH50); protease activities; and total Immunoglobulin (IgM) improved significantly (P < 0.05), particularly in the T2 group. The T2 group also demonstrated the highest survival rate among all groups. The present findings indicate that dietary administration of inulin promotes growth and biochemical parameters, as well as serum immunity and mucosal immune responses of rainbow trout, in which a 2% inclusion produced the best results.