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  • Author: Guo-Yu Weng x
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Chih-Li Chen, Tien-Pen Hsu and Guo-Yu Weng

Abstract

In this paper two new approaches are developed to calculate the astronomical vessel position (AVP). Basically, determining the AVP is originated from the spherical equal altitude circles (EACs) concept; therefore, based on the Sumner line's idea, which implies the trial-and-error procedure in assumption, the AVP is determined by using the two proposed approaches. One consists in taking the great circle of spherical geometry to replace the EAC to fix the AVP and the other implements the straight line of the plane geometry to replace the EAC to yield the AVP. To ensure the real AVP, both approaches choose the iteration scheme running in the assumed latitude interval to determine the final AVP. Several benchmark examples are demonstrated to show that the proposed approaches are more accurate and universal as compared with those conventional approaches used in the maritime education or practical operations.

Open access

X. Yu, Y. Guo, X. Zhang, F. Li, Q. Weng, M. Li and S. Gan

Abstract

A suite of 91 expressed sequence tag (EST) derived cleaved amplified polymorphic sequence (CAPS) markers were developed and used for enriching the genetic maps of Eucalyptus urophylla and E. tereticornis built previously based on random amplified polymorphic DNA (RAPD) markers. The EST-CAPS markers were highly similar to original ESTs, with sequence identity ranging from 92.5% to 100.0%. In linkage analysis, 48 and 42 EST-CAPSs were integrated into the genetic maps of E. urophylla and E. tereticornis, respectively, including 13 shared by both maps, while 14 were unmapped. For E. urophylla, the final map had a total length of 1789.5 cM and a mean interval between markers of 9.7 cM, being 284.9 cM larger and 1.3 cM less than those of the prior RAPD map, respectively. For E. tereticornis, the final map had a length of 1488.1 cM and a mean interval of 10.3 cM, being 452.4 and 0.2 cM more than the prior map, respectively. All the 77 newly mapped EST-CAPSs found each at least one homologue in the E. grandis genome sequence released recently, and conserved synteny and colinearity were observed between E. grandis genome and our linkage groups. The enriched maps would provide a set of useful markers for genome analysis, comparative mapping and fine-mapping of important genes located in conserved regions for the important tree genus Eucalyptus.

Open access

Jianhui Yang, Yu Ren, Zhong-Guan Lou, Xue Wan, Guo-Bin Weng and Dong Cen

Abstract

Bladder cancer (BCa) is one of the most common urinary cancers. The present study aims to investigate whether Paeoniflorin (Pae) can exert inhibitory effects on BCa. The results showed that Pae inhibited proliferation of human BCa cell lines in a concentration- and time-dependent manner. Pae and cisplatin (Cis) synergistically inhibited the growth of tumours in RT4-bearing mice. Pae treatment neutralized the body loss induced by Cis. Moreover, Pae induced apoptosis in RT4 cells and increased the activities of caspase3, caspase8 and caspase9. Western blotting and immunohistochemical analysis revealed that the phosphorylated signal transducer and activator of transcription-3 (p-STAT3) level were decreased in Pae-treated RT4 cells and Pae-treated tumour-bearing mice. Furthermore, STAT3 transcriptional target B-cell lymphoma-2 was decreased in Pae-treated RT4 cells. Interestingly, Pae prevented translocation of STAT3 to the nucleus in RT4 cells. Collectively, Pae inhibits the growth of BCa, at least in part, via a STAT3 pathway.