Aleksandra Anđelković, Nada Barac and Goran Milovanović
Supply chain assumes a unique business process without inter-company boundaries. Each supply chain partner is important for achievement of customer requirements. On the one hand, supply chain is an inexhaustible source of competitive advantage, created by the companies, which constitute it. However, on the other hand, due to its complexness, supply chain is an easy target of numerous factors, which jeopardize its competitiveness. Some of those factors are: turbulence, deliberate threats, external pressures, resource limits, sensitivity, and supplier/customer disruptions. Successful dealing with those factors assumes establishment of mechanisms for increasing supply chain resilience. Many researches indicate that some factors have greater influence on supply chain disruption and discontinuance. If those factors can be isolated, it will be easier to manage them or to avoid their influence. Therefore, the objective of the research presented in this paper is identification of the most frequent causes of supply chain disturbances, as a first step for developing supply chain resilience. According to the research results, the authors suggest what should be a primary focus of supply chain management, dealing with issues of resilience. Also, the authors indicate which characteristics (capability factors) supply chains have to have in order to be considered resistant.
Ana Banko, Ivana Lazarevic, Goran Stevanovic, Andja Cirkovic, Danijela Karalic, Maja Cupic, Bojan Banko, Jovica Milovanovic and Tanja Jovanovic
Background: Primary Epstein-Barr virus (EBV) infection is usually asymptomatic, although at times it results in the benign lymphoproliferative disease, infectious mononucleosis (IM), during which almost half of patients develop hepatitis. The aims of the present study are to evaluate polymorphisms of EBV genes circulating in IM isolates from this geographic region and to investigate the correlation of viral sequence patterns with the available IM biochemical parameters.
Methods: The study included plasma samples from 128 IM patients. The genes EBNA2, LMP1, and EBNA1 were amplified using nested-PCR. EBNA2 genotyping was performed by visualization of PCR products using gel electrophoresis. Investigation of LMP1 and EBNA1 included sequence, phylogenetic, and statistical analyses.
Results: The presence of EBV DNA in plasma samples showed correlation with patients’ necessity for hospitalization (p=0.034). The majority of EBV isolates was genotype 1. LMP1 variability showed 4 known variants, and two new deletions (27-bp and 147-bp). Of the 3 analyzed attributes of LMP1 isolates, the number of 33-bp repeats less than the reference 4.5 was the only one that absolutely correlated with the elevated levels of transaminases. EBNA1 variability was presented by prototype subtypes. A particular combination of EBNA2, LMP1, and EBNA1 polymorphisms, deleted LMP1/P-thr and non-deleted LMP1/P-ala, as well as genotype 1/ 4.5 33-bp LMP1 repeats or genotype 2/ 4.5 33-bp LMP1 repeats showed correlation with elevated AST (aspartate aminotransferase) and ALT (alanine transaminase).
Conclusions: This is the first study which identified the association between EBV variability and biochemical parameters in IM patients. These results showed a possibility for the identification of hepatic related diagnostic EBV markers.