The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury.
Material and Methods
Eighteen miniature pigs were randomly divided into three groups: a sham operated group (sham group, laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), and a hydrogen-rich saline intervention group (IRI + HRS group). Samples of hepatic tissue and serum were collected at the time of reperfusion and then 3 h, 1 d, and 3 d post reperfusion. Liver function, oxidative stress, autophagy-related mRNA genes, and protein expression were evaluated. Changes in cell and tissue ultrastructure were examined by transmission electron microscopy.
Compared with the sham group, the level of autophagy of hepatocytes increased in the IRI and IRI + HRS groups, corresponding to high oxidative stress and severe liver function injury. Liver function, antioxidant content, autophagy levels, and liver injury were improved after intervention with HRS in the IRI + HRS group compared with the IRI group.
Intervention with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy.
The miniature pig possesses unmatched advantages as an animal model because of its high homology with humans. Our experiment aimed to build a chronic renal failure (CRF) model in pigs via laparoscopy.
Material and Methods
Laparoscopic surgery was performed twice to build a CRF model. The first surgery was a left partial nephrectomy and the second was a right radical nephrectomy. Pigs were grouped by the total renal tissue to be resected: ⅔, ¾ or ⅚. Physiological parameters (rectal temperature and heart rate), haematological parameters (WBC and RBC) and renal function (serum creatinine – CR and blood urea nitrogen – BUN) were measured preoperatively and every week postoperatively.
After renal resection the pigs manifested chronic renal failure. Heart rate and body temperature declined to varying degrees over 12 postoperative weeks. No significant difference was observed between the different groups. The result of renal function tests found that postoperative serum CR and BUN in all groups were continuously elevated, and the level of serum CR at two weeks post procedure differed very significantly from its preoperative value (P < 0.05). BUN was significantly elevated at one week (P < 0.05). The renal function decreased significantly faster in the ⅚ group than in the other two groups. The trend of renal function change was similar among groups, but progress was slower in the ⅔ and ¾ groups.
⅚ kidney resection was the optimal miniature pig model of CRF.