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  • Author: Fan Zhang x
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Open access

Haiyan Wang, Lei Zhang, Shuiyuan Cheng, Fan Feng and Wei Liu

Open access

John Zhang, Ming Fan, Bin Gu, Vijay Mukherjee, Bin Zhang and J. Leon Zhao

Open access

Yue-qiu Zhang, Shao-xia Xu, Sai-nan Bian, Li-fan Zhang, Yao Zhang, Wei-hong Zhang, Ying-chun Xu and Xiao-qing Liu

Abstract

Objective To investigate the infection rate of hepatitis C virus among the ambulatory patients and in-patients of a tertiary teaching hospital, and study the demographic factors related to the prevalence of hepatitis C virus infection.

Methods All patients tested for hepatitis C virus antibody from July 2008 to July 2009 in Peking Union Medical College Hospital were enrolled in this cross-sectional analysis. The prevalence of hepatitis C virus infection was compared according to age, gender, and departments, respectively. Among patients with positive serology hepatitis C virus marker, the positivity of hepatitis C virus RNA was analysed.

Results Among 29 896 subjects included, the hepatitis C virus antibody of 494 patients were positive (1.7%). When patients were divided into 9 age groups, the age specific prevalence of hepatitis C virus antibody were 0.2%, 1.7%, 1.2%, 1.1%, 1.5%, 1.9%,2.6%, 2.4% and 2%, respectively. The prevalence of hepatitis C virus antibody in non-surgical department and surgical department was 3% and 1%, respectively. The prevalence of hepatitis C virus antibody of males was higher than that of the females. Total of 194 patients with positive hepatitis C virus antibody were tested for hepatitis C virus RNA, the RNA level of 113 patients (58.2%) were higher than the low detection limit.

Conclusions The prevalence of hepatitis C virus antibody was relatively high among patients of general tertiary hospital. Age group of 60-69, males and patients in non-surgical departments were factors associated with high rate of hepatitis C virus infection.

Open access

Jian Fan, Li Zhang, Qi long Wang and Hui Lin

Abstract

Background: Metastasis is responsible for most cancer-related death, and the metastatic spread of neoplastic cells may be related to the ability of migration and invasion. Chemokine receptor 9 (CCR9) plays an important role in cutaneous melanoma and prostate cancer cells migration and invasion.

Objective: Investigate the specific role of the chemokine-ligand (CCR9-CCL25) axis in the development of nonsmall cell lung cancer (NSCLC) metastasis.

Methods: Semi-quantitative reverse transcriptase-PCR, western-blot, flow cytometry, migration, and invasion assays were used to examine the function of CCR9 in the NSCLC cells.

Results: CCR9 was highly expressed in NSCLC patient cancer tissue. In addition, in vitro migration and invasion studies on human bronchial epithelial cells of the BEAS-2B and human squamous lung cancer cell lines NCI-H157 showed that migration in response to the CCL25 was inhibited by CCR9 antibody.

Conclusion: CCR9 might play an important role in the migration and invasion of the NSCLC cells.

Open access

Dansheng Lei, Guanhui Zhang, Jian Fan, Jing Yu and Shaoqing Hu

Abstract

Background: Ficolins are lectins that have been demonstrated to play an important role in innate immune response in a variety of diseases. Mycobacterium tuberculosis (M.tb) infection can trigger a series of changes in the host. However, the role of ficolin in tuberculosis is still unclear.

Objective: We investigated the expression of ficolin in peripheral blood mononuclear cell (PBMC) in TB patients and healthy control.

Methods: Using semi-quantitative RT-PCR, western blotting, and flow cytometry, we compared the expression of M-ficolin, L-ficolin, and H-ficolin in the peripheral blood mononuclear cell (PBMC), purified monocytes, and cultured dendritic cells of TB patients with healthy volunteers as controls.

Results: M-ficolin expression in PBMC was significantly lower at both mRNA and protein levels in TB patients as compared to healthy controls. The lower M-ficolin level in TB patient PBMCs may be attributed to its lower level in monocytes. The expression levels of H-ficolin and L-ficolin in both healthy controls and TB patients were very low and they had no significant differences between the two groups.

Conclusions: Compared to healthy controls, M-ficolin expression is significantly lower in TB patients. Measurement of M-ficolin may be a potential auxiliary tool to diagnose TB infection.

Open access

Min Zhang, Shidong Fan, Hanhua Zhua and Sen Han

Abstract

The study of the flow characteristics of the solid-fluid two phase flow in the cutter suction dredger is very important for exploring the slurry formation mechanism and optimizing the operational parameters. In this study, standard k-ε model and Multiple Reference Frame are applied to numerically simulate flow field in and around the cutting system, then with the steady convergent result of the simulation as the initial condition, Discrete Phase Mode is used to solve the particle motion equation by fully coupling the continuous phase and the particles. The influence of suction flow velocity and cutter’s rotating speed on particles suction are analyzed, and effectively suctioned particles numbers are also quantitatively studied. The simulation result shows that the DPM model is able to simulate the movement of particles in and around the cutter suction dredger’s cutting system, in the fluid flow filed velocity vector and pressure distribution on different planes show different characteristics, and under higher suction velocity and lower cutter rotating speed more particles are suctioned into the suction inlet. The results can help better understand flow characteristics of solid-fluid 2-phase-flow of cutter suction dredger’s cutting system, and provide theoretical support for relative system design and operational parameters optimization.

Open access

Xianglin Zhang, Fangfang Fan, Yong Huo and Xiping Xu

Open access

Pei-yan Zhang, Si-min Yao, Fan Zhang, Ming-xia Zhang, Wei Li, Jing Yuan, Xin-chun Chen and Bo-ping Zhou

Abstract

Objective Various immune cells in patients with CHB have been demonstrated to play critical roles in HBV infection. The goal of this study is to observe changes in Th17, Treg, Th1 and B lymphocytes from peripheral blood and to evaluate immune status of CHB patients undergoing antiviral treatment.

Methods Total of 49 CHB patients, 19 asymptomatic carriers and 29 healthy donors were included in our present study. The frequencies of peripheral Th17 cells (CD3+CD4+IL-17+Tcells), Treg cells (CD3+CD4+CD25+CD127- T cells), Th1 cells (CD3+CD4+IFN-γ T cells) and B lymphocytes in chronic hepatitis B (CHB) were analyzed by flow cytometry.

Results The frequency of Th17 cells increased after treatment for 6 months, but there was no statistically significant difference of IL-17 expression between baseline and 6 months after treatment. The frequencies of Treg cells, momory B cells and total CD19+ B cells decreased after antiviral treatment. The frequencies of Th1 cells and plasma cells increased after antiviral treatment.

Conclusions This study highlights that the reestablishment of immune function during antiviral treatment in CHB patients, which caused by the antiviral drugs or the patients themselves. CHB patients may exhibit varied responses to these antiviral drugs. It is essential to supplement immune therapy during the antiviral treatment, but Th17 may play a limited role in inflammation during antiviral treatment, targeting Th17 therapy may not be useful for CHB treatment. More time and more experiments are critical to explain it.