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  • Author: Ewa Maj x
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Abstract

Two successful women, Jacqueline Kennedy and Lady Bird Johnson, influenced and were in turn influenced by the political careers of their husbands. An analysis of their oral histories, Jacqueline Kennedy: Historic Conversations on Life with John F. Kennedy (released in 2011) and Lady Bird Johnson: An Oral History (2012), demonstrates that John F. Kennedy and Lyndon Johnson became the first men of the American nation not only through their personal virtues, but also through the influence of their wives and who had a significant impact on their careers. Granted, both Jackie and Lady Bird extolled their husbands’ merits, stressing that they were “only” their wives; however, both First Ladies played an essential diplomatic and political role, ensuring their husbands’ physical and emotional well-being in private and public life. The article demonstrates how Jacqueline Kennedy and Lady Bird Johnson promoted the positive images of their husbands’ terms in office via their oral histories and continued to do so after their deaths. Moreover, the article considers some important differences between the two histories. Jacqueline did not edit her previously authorized interviews, whereas Lady Bird made important changes to hers; furthermore, in direct contrast to Lady Bird, Jacqueline never wrote a memoir or autobiography, which makes her oral history the most valuable source on her views about her life and her husband’s career.

Abstract

The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at these temperatures. Significantly more mortality cases were reported in the redfin perch population, reaching a maximum of 24% compared with 12% in the rainbow trout group at 20-22°C. EHNV was reisolated from redfin perch and rainbow trout tissue in cell culture and the infection was confirmed by a molecular method and histopathology during the duration of the experiment. This study revealed that fish from Polish farms can be susceptible to EHNV even at lower temperatures.

Abstract

The aim of the study was to identify the genotype of Polish isolates of salmonid alphaviruses (SAV) and to find the origin of the virus. Samples for virus isolation included the kidneys, spleen, and liver pooled from 10 fish. A typical cytopathic effect was observed after inoculation of samples on cell lines. Total RNA was extracted from cell culture supernatant and submitted to RT-PCR with primers amplifying two informative regions of the genome: a conserved region in the E2 gene and a variable region in the nsP3 gene. The sequences revealed that the strain from Poland belonged to subtype SAV 2, indicating a very strong genetic identity with isolates from Italy and France.

Abstract

Introduction

Koi herpesvirus (KHV) has infected farmed common carp in Poland clinically and asymptomatically since 2004. The role of non-carp species as vectors of virus transmission is well known except for in the case of KHV. The aim was to better understand this virus’ infection and transmission pathways in common carp, looking at the potential vector role of fishes kept with them.

Material and Methods

Eight species were experimentally infected with KHV by immersion in a suspension at 20°C ±1 and transferred to a tank after 45 minutes. Specimens were euthanised at intervals up to 56 days post infection (dpi) and tissue was examined for KHV DNA. Surviving infected fishes were introduced at intervals, each time into a separate tank, to naïve common carp for experimental infection. These were observed daily for symptoms, sacrificed along with controls after three months, and dissected to provide tissue samples. Also fish from 14 species collected from a farm with a history of KHV were sampled from 3 to 22 months after disease was confirmed. Organ sections from single fish were collected in a single tube.

Results

Viral DNA was detected in tench and roach samples up to 49 dpi, but in three-spined stickleback and stone maroko samples only up to 14 dpi. Transmission of KHV to naïve carp occurred after cohabitation. KHV DNA was detected in three fish species three months after the farm outbreak.

Conclusion

We confirmed that grass and Prussian carp, tench, roach, and brown bullhead can transfer the virus to naïve common carp.