Search Results

You are looking at 1 - 4 of 4 items for

  • Author: Ewa Kamińska x
Clear All Modify Search
Open access

Renata Wojciechowska, Ewa Hanus-Fajerska, Iwona Kamińska, Aleksandra Koźmińska, Olga Długosz-Grochowska and Anna Kapczyńska

Abstract

The southern African geophyte Lachenalia with an enormous number of species and cultivars is nowadays a commercially important plant material. There is a need for research on the optimization of growing conditions to obtain a satisfactory visual quality of potted plants, which may boost its production on the international ornamental market. Our research can be considered as an innovative study on supplemental irradiation with various light spectra in relation to flower quality of Lachenalia spp. The main objective was to examine the usefulness of LED lighting in extending the length of the natural day to a 16/8 h photoperiod in order to control the development of Lachenalia ‘Rupert’ inflorescence during greenhouse cultivation in Central-European winter time. Three light treatments were applied with red (660 nm) and blue (440 nm) light in different ratios: 100% red (100/0), 90% red mixed with 10% blue (90/10) and 80% red with 20% blue (80/20). The PPFD at the plant leaf level was approx. 150 μmol m-2 s−1. The most favourable spectrum, 90/10, induced the longest inflorescences characterized by the highest stem diameter with simultaneously the highest number of florets. Additionally, blue light increased the anthocyanin content in the corolla by about 35%, compared with plants exposed to 100% red light and non-irradiated ones (control plants). This first study on the wavelength ratios is aimed to increase the production quality of Lachenalia and indicates the need for continuation.

Open access

Artur Jóźwik, Ewa Polawska, Nina Strzałkowska, Krzysztof Niemczuk, Małgorzata Łysek-Gładysińska, Agnieszka Kamińska and Monika Michalczuk

Abstract

The aim of the study was to assess the activity of lysosomal enzymes: aminopeptidases, including alanine aminopeptidase (AlaAP), leucine aminopeptidase (LeuAP), arginine aminopeptidase (ArgAP), and glycosidases, such as β-galactosidase (BGAL), β-glucuronidase (BGRD), β-glucosidase (BGLU), N-acetyl-β-hexosaminidase (HEX), α-glucosidase (AGLU) and α-mannosidase (MAN) in the liver of ostriches (n = 80) fed diet supplemented with linseed (4% and 8%) and rapeseed (5% and 10%), with low and high level of vitamin E. (40 and 100 mg). The results indicate that higher level of vitamin E or 4% linseed supplementation in ostrich diet generally increase the activity of glycosidase enzymes and decrease the activity of aminopeptidases in the liver. The 8% linseed and rapeseeds feeding in decreased the activity of AlaAP, LeuAP, and ArgAP and increased only the activity of BGLU.

Open access

Michał Szulc, Piotr Mularczyk, Patryk Grządzielski, Przemysław Zakowicz, Radosław Kujawski, Agnieszka Gryszczyńska, Waldemar Buchwald, Artur Teżyk, Anna Krajewska-Patan, Ewa Kamińska and Przemysław Ł. Mikołajczak

Summary

Introduction: Rhodiola rosea (RR) and Rhodiola kirilowii (RK) are well known for their influence on central nervous system, however their impact on the development of alcohol tolerance has not yet been proven.

Objective: The aim of this study was to determine the ability of RR and RK roots extracts to inhibit the development of alcohol tolerance in vivo, both, peripheral (metabolic) and central ones.

Methods: Male Wistar rats were treated with RR and RK extracts (p.o.) and ethanol (i.p.) for ten consecutive days. On the first, third, fifth and eighth days the hypothermic action of ethanol was measured, while on the ninth day the loss of righting reflex was examined. On the tenth day rats were treated with assigned extract and sacrificed 1 h after the ethanol injection.

Results: Both extracts inhibited development of tolerance to the hypothermic action of ethanol. The observed effect seems to be specific since none of the extracts affected body temperature in water-treated animals. RK extract also prolonged the hypnotic action of ethanol. RR-treated rats had higher blood-ethanol concentrations, in contrast to RK ones.

Conclusions: RR and RK extracts inhibited the development of tolerance to the hypothermic action of ethanol. Prolongation of the hypnotic action of ethanol by RK extract may be associated with influence on the central nervous system, while the RR one also inhibited the development of metabolic tolerance.

Open access

Michał Szulc, Piotr Mularczyk, Radosław Kujawski, Agnieszka Gryszczyńska, Ewa Kamińska, Bogna Geppert, Justyna Baraniak, Małgorzata Kania-Dobrowolska, Marcin Ożarowski, Anna Krajewska-Patan and Przemysław Ł. Mikołajczak

Summary

Introduction: In recent years, the search for potential neuroprotective properties of salidroside and its ability to influence the activity of nervous system become the subject of intense studies of many research groups. None of these studies, however, include an attempt to determine the effect of salidroside on the course of alcohol tolerance in vivo.

Objective: The aim of this study was to investigate the ability of salidroside to inhibit the development of alcohol tolerance in rats, determining whether the effect of its action may occur in a dose-dependent manner, reducing both metabolic and central tolerance without affecting body temperature in control rats.

Methods: Male Wistar rats were injected daily with ethanol at a dose of 3 g/kg for 9 consecutive days to produce ethanol tolerance. Salidroside in two doses (4.5 mg/kg and 45 mg/kg b.w.) or vehiculum was administered orally. On the 1st, 3rd, 5th and 8th day a hypothermic effect of ethanol was measured, while the loss of righting reflex procedure was performed on the 2nd, 4th, 6th and 7th day. On the 9th day rats were treated with salidroside, sacrificed 1 h after ethanol injections and blood was collected for blood-ethanol concentration measurement.

Results: Salidroside at a dose of 45 mg/kg inhibited the development of tolerance to hypothermic and sedative effects of ethanol, whereas insignificant elevation of blood-ethanol concentration was observed. The dose of 4.5 mg/kg b.w. had minimal effect, only small inhibition of tolerance to hypothermic action was observed. Salidroside affected neither body mass growth nor body temperature in non-alcoholic (control) rats.

Conclusions: Results of the study indicate that salidroside at a dose of 45 mg/kg inhibited the development of tolerance to the hypothermic effect of ethanol. Observed inhibition of tolerance to the sedative effect of ethanol seems to be associated with salidroside influence on the central nervous system. A comprehensive explanation of the abovementioned observations requires further pharmacological and pharmacodynamic studies.