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Ewa Borzym and Joanna Maj-Paluch

Abstract

The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at these temperatures. Significantly more mortality cases were reported in the redfin perch population, reaching a maximum of 24% compared with 12% in the rainbow trout group at 20-22°C. EHNV was reisolated from redfin perch and rainbow trout tissue in cell culture and the infection was confirmed by a molecular method and histopathology during the duration of the experiment. This study revealed that fish from Polish farms can be susceptible to EHNV even at lower temperatures.

Open access

Ewa Borzym, Joanna Maj-Paluch, Magdalena Stachnik, Marek Matras and Michał Reichert

Abstract

The aim of the study was to identify the genotype of Polish isolates of salmonid alphaviruses (SAV) and to find the origin of the virus. Samples for virus isolation included the kidneys, spleen, and liver pooled from 10 fish. A typical cytopathic effect was observed after inoculation of samples on cell lines. Total RNA was extracted from cell culture supernatant and submitted to RT-PCR with primers amplifying two informative regions of the genome: a conserved region in the E2 gene and a variable region in the nsP3 gene. The sequences revealed that the strain from Poland belonged to subtype SAV 2, indicating a very strong genetic identity with isolates from Italy and France.

Open access

E. Borzym, T.A. Karpińska and M. Reichert

Abstract

Ranavirus was detected in adult sheatfish, with clinical signs, on a Polish fish farm in February. Farm isolates induced a strong cytopathic effect in vitro and were identified by electron microscopy and PCR amplification of the ranavirus specific gene fragment. Restriction analysis with the Acc I enzyme showed that isolated ranaviruses were different from the epizootic heamatopoietic necrosis virus (EHNV). We sequenced a fragment of the major capsid protein (MCP) gene and found that isolates were similar to other strains of ranaviruses, available in GenBank.