The aim of this study was to determine the enzymatic activity (dehydrogenase and urease) in trophically diverse Brunic Arenosols and Cambisols. Efforts to establish the relationship between enzymatic activity and physico-chemical properties of various subtypes of Brunic Arenosols and Cambisols were attempted. Another aim was to determine the effect of vegetation on the properties of soil surface levels. The study was conducted on 94 plots located in nature reserves and national parks in the Polish lowland area. Dehydrogenase activity and urease showed large variations in the subtypes of the distinguished Brunic Arenosols and Cambisols. Dehydrogenases and urease activity in surface layers of fresh humus of Cambisols and Brunic Arenosols was strongly associated with the plants. This is confirmed by the relationship between the enzymatic activity and the type of accumulated humus substances, the ratio of carbon to nitrogen and humus horizons reaction.