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Andra Costache, Silvana Angelescu, Doina Barbu, Elena Popescu, Delia Ileana Mut Popescu and Anca Roxana Lupu

Abstract

Patients with acute leukemia develop abnormalities of haemostasis, leading not only to bleeding, but also to thrombotic complications. The pathogenesis of these complications is complex and multifactorial. Because platelets and platelet derived microparticles are key players in haemostasis and thrombosis, we presumed their roles in the prediction of bleeding and thrombotic complications. Our study groups included 24 patients with acute leukemia and 16 healthy volunteers. Platelet aggregation evaluation was performed by impedance whole blood aggregometry and the ennumeration of platelet derived microparticles was done by means of flow cytometry. Eight patints developed hemorrhagic complications associated with reduced platelet aggregation response at hospital admission. Major thrombotic events occurred in 5 patients, being preceded by increased platelet aggregation in 3 cases and high level of platelet derived microparticles in 2 cases. Our findings reveal that whole blood platelet aggregometry could be a valuable tool especially in the detection of platelet hyperreactivity and in the prediction of thrombotic events. A high level of platelet derived microparticles could also predict thrombosis. These hypotheses need further evaluation and confirmation on larger number of patients.

Open access

Cristina Popescu, Anca Leuştean, Alina Elena Orfanu, Codruţa Georgiana Carp and Victoria Aramă

Abstract

Introduction: Streptococcal infection can be responsible for multiple complications, such as toxic, septic or allergic disorders. Toxin-mediated complications (TMC) can appear during the acute phase of disease and can involve any organ, causing carditis, arthritis, nephritis, hepatitis etc.

Case presentation: The case of a young woman without a history of recurrent streptococcal tonsillitis, admitted to “Matei Balş” National Institute for Infectious Diseases, Bucharest, Romania, presenting with fever, sore throat and exudative tonsillitis, is detailed. The initial test for Streptococcus pyogenes was negative. The patient had leukopenia with severe neutropenia, high values of inflammatory biomarkers and electrocardiographic (ECG) changes with inverted T waves in leads V1-4 and flattened T waves in V5-6. There were no other cardiac signs or symptoms. The patient received cefuroxime for two days. On admission, the patient was prescribed Penicillin G and dexamethasone which resulted in a rapid recovery. The leucocytes count returned to normal as did the ECG abnormalities. At the time of discharge, the antistreptolysin O titre was high.

Conclusions: The case highlights the toxin-mediated complications (TMC) of streptococcal infection which occur from the outset of the disease.

Open access

Mariana Popescu, Florin Oancea, Elena Radu, Mălina Deșliu-Avram and Călina Petruța Cornea

Abstract

Natural by-products from vegetable oil industries and spent edible oils from domestic or public food spaces should be recycled to obtain new added value products. Present paper proposed a technical solution for complete valorisation of inedible oilseeds or spent edible oils into bioproducts for nutrition and protection of plants cultivated in conservative organic agrosystems. Pressed cakes resulted from mechanical cold extraction of mustard oil contain residual oil and bioactive compounds which were released using an enzymatic cocktail 1:1 cellulase with proteases coupled with azeotropic solvents into a single Soxhlet extractor. From mustard meal, a solid fraction with glycerol derivatives of fatty acids (56.23% oleate and 17.47% linoleate) decanted from syrup (41.78% xylopyranoside and 48.48% trilinolein) and from mustard cake (76.44% linoleate) in the supernatant, the same oligosaccharide (29.64%) and proteinates (30.18%) in the solid fraction. The total extract was simultaneously concentrated and converted into a bioactive potassium salt emulsion able to encapsulate insectofungicidal natural compounds as bioproducts with agronomical applications.

Open access

A. I. Tucmeanu, Delia Mioara Popescu, Elena Valentina Ionescu, Carmen Oprea, A. Mociu and Mădălina Gabriela Iliescu

Abstract

Health management consultancy has become an important source of innovation in management, a bridge between the academic environment, companies and opinion-makers in the field. The objective of this work is to identify the success factors of a consultancy mission within the health system, with the proposal of direct investigation of the hypothesis regarding the positive association between the success of consultancy missions and the consultant’s professional integrity. The survey was carried out during January and February 2017, in Bucharest health units, by applying questionnaires to 68 managers both in the public and private system. The analyzed parameters were: the consultant’s professional integrity, the consultant's competency, the relations with the consultant, the correct definition of the mission, available financial resources, term observance, and related costs. The information obtained was statistically processed using the SPSS system. The answer options to the items in the questionnaire allowed statistical correlations, verifying the survey hypothesis regarding the association between success and professional integrity. Any consultancy mission entails negotiation skills from the consultant, as well as an open attitude, listening and communications skills. The environment created by the manager can even further influence the change acceptance.

Open access

Adriana Sireteanu, Roxana Popescu, Elena Emanuela Braha, Cornel Bujoran, Lăcrămioara Butnariu, Lavinia Caba, Elena Graur, Eusebiu Vlad Gorduza, Mihaela Grămescu, Iuliu Cristian Ivanov, Monica Pânzaru and Cristina Rusu

Abstract

Intellectual disability (ID) is a common disorder, with major consequences for individual, family and society. Due to clinical and genetic heterogeneity of ID, in about 50% of cases an etiologic diagnosis cannot be established. The aim of this study was to evaluate the ability of a combination of MLPA kits to establish the diagnosis in 369 patients with syndromic ID and normal or uncertain routine karyotype results. All patients were assessed for chromosome imbalance using SALSA MLPA P064 or P096 kits, if the phenotype was suggestive of a microdeletion syndrome (subgroup A - 186 patients), or subtelomeric P036 and P070 kits, if the phenotype was not suggestive of a microdeletion syndrome or if the result of the standard karyotype was uncertain (subgroup B - 183 patients). Abnormal results detected by these kits were further characterized using appropriate follow-up MLPA kits (Telomere Follow-up set, P029-A1, P250-B2, ME028-B1). In subgroup A we identified 25 patients with microdeletions (13.4%). Using subtelomere screening and follow-up kits in subgroup B we detected cryptic rearrangements in 7.5% cases and identified the origin of the unknown material noticed in the standard karyotype in 10 out of 11 patients. Summarizing data from the two groups, the combined use of MLPA kits led to the diagnosis in 10.6% (38/358) patients with normal karyotype. Using follow-up MLPA kits allowed us both to confirm abnormalities and to determine their size, which facilitated the interpretation of the clinical significance of these rearrangements. For laboratories that do not have yet access to microarray technology, using several MLPA kits represents an effective strategy for establishing the diagnosis in ID patients.

Open access

Carmen Popescu, Dorel Ureche, Camelia Ureche and Elena Nechita

Abstract

Fish represents an important food source for people worldwide. Moreover, although considered a very old occupation, fishing continues to provide jobs, especially for the people living in the coastal countries. The quality of surface waters affects the quality of fish as a food source. For this reason, the present study aims to assess the quality of the ichthyofauna in the Oituz River and some of its tributaries using several parameters that have been computed based on the biometric data of the biological material gathered during 2004-2008, in correlation with the water pH and water temperature. The present paper also highlights some observations regarding the changes of the analyzed ecosystems, as well as some recommendations regarding the fish consumption in the studied basin, considered as a food source for humans.

Open access

Cristina Rusu, Adriana Sireteanu, Lăcrămioara Butnariu, Monica Pânzaru, Elena Braha, Doina Mihăilă and Roxana Popescu

Abstract

Duchenne and Becker muscular dystrophies (DMD/BMD) are X-linked progressive muscle disorders determined by mutations of the dystrophin (DMD) gene. Multiplex Ligation - Dependent Probe Amplification (MLPA) is a simple, inexpensive and reliable test for molecular diagnosis of DMD gene mutations. It identifies exonic copy number variations in the DMD gene, but the test should be completed with sequencing analysis in case of single exon deletions/duplications. The aim of this study was to evaluate the efficiency of MLPA as a DMD mutation screening tool in affected males and carrier females, as well as to appreciate the frequency of different types of mutations and to check the validity of the “reading frame rule”. We have used MLPA for the detection of deletions/ duplications in DMD gene in 53 individuals (30 affected males and 23 asymptomatic female relatives) referred for evaluation and genetic counseling due to the clinical suspicion of DMD/BMD. In the affected males (21 DMD and 9 BMD) MLPA had a detection rate of 63.5% (53.5% deletions and 10% duplications). The most frequently deleted exon was exon 45 and the most frequent duplication involved exons 3-5, confirming the presence of the two hotspot mutation regions reported in the literature. Mutations detected in our study have a slightly different location compared to literature data. Reading frame rule was valid in 84% of our cases.

Open access

Florina Violeta Anghelina, Dan Nicolae Ungureanu, Carmen Popa, Elena Valentina Stoian, Ileana Nicoleta Popescu, Cristiana Maria Enescu and Constantin C. Anghelina

Abstract

In this presentation are evidenced instrumental characterization by Electronic Scanning Microscopy (SEM) equipped with EDS / WDS of aluminum alloys such 2024 alloy with multiple synergistic purposes as: acquisition of new knowledge, increase the amount of information obtainable about the material, increasing the efficiency and the quality of the tests, characterization of special destination alloys and assessing their conformity with the specified requirements. In this direction, it requires both the knowledge the test methods (theoretical basis, test method, procedure relating to the method, quality assurance testing) as well material characteristics investigated. These aspects are necessary for test system design, and preliminary interpretation of experimental results.

Open access

Elena-Daniela Grigorescu, Cristina Mihaela Lăcătuşu, Gina Eosefina Botnariu, Raluca Maria Popescu, Alina Delia Popa, Alina Onofriescu and Bogdan-Mircea Mihai

Abstract

The physician-patient communication has an essential role in establishing and supporting the relationship between these two partners. Moreover, modern medicine highlights the patient-centered approach. Publications assessing the impact of an efficient physicianpatient communication on medical care results in diseases such as diabetes and hypertension have revealed a positive correlation between patient’s satisfaction about the communication with the physician and values of blood pressure, glycated hemoglobin and pain intensity. Interventions needed in both doctors and patients for developing communication abilities were paid special attention in order to achieve an appropriate improvement in their communicative interaction during periodical appointments. In the field of diabetes mellitus, the medical challenge is to improve patients’ knowledge about medical care; this aim is achieved only by therapeutic education, using high-quality communication techniques.

Open access

Alina Elena Orfanu, Cristina Popescu, Anca Leuștean, Anca Ruxandra Negru, Cătălin Tilişcan, Victoria Aramă and Ștefan Sorin Aramă

Abstract

Sepsis represents a severe pathology that requires both rapid and precise positive and differential diagnosis to identify patients who need immediate antimicrobial therapy. Monitoring septic patients′ outcome leads to prolonged hospitalisation and antibacterial therapy, often accompanied by substantial side effects, complications and a high mortality risk. Septic patients present with complex pathophysiological and immunological disorders and with a predominance of pro-inflammatory or anti-inflammatory mediators which are heterogeneous with respect to the infectious focus, the aetiology of sepsis or patients′ immune status or comorbidities. Previous studies performed have analysed inflammatory biomarkers, but a test or combinations of tests that can quickly and precisely establish a diagnosis or prognosis of septic patients has yet to be discovered. Recent research has focused on re-analysing older accessible parameters found in the complete blood count to determine the sensitivity, specificity, positive and negative predictive values for the diagnosis and prognosis of sepsis. The neutrophil/lymphocyte count ratio (NLCR), mean platelet volume (MPV) and red blood cells distribution width (RDW) are haemogram indicators which have been evaluated and which are of proven use in septic patients′ management.