Innovation is not a simple concept but is the main source of success. It is more important to have the right people and mindsets in place than to have a perfectly crafted plan in order to make the most out of an idea or business. The aim of this paper is to emphasize the importance of intangible assets when it comes to machine vision inspection of thermoplastic materials pointing out some aspects related to knowledge based assets and their need for a success idea to be developed in a successful product.
Isabela Popa, Diana Protasiewicz, Cristina Muntean, Simona Georgiana Popa and Maria Mota
Phisical activity, regularly performed, give us a lot of health benefit, especially inpreventing cardiovascular disease, diabetes mellitus (DM) and obesity. Physicalexercise, defined as a controlled, progressive, supervised, requires muscular activity,involving energy consumption through metabolic and thermoregulatory processes. Itcan be classified as aerobic and anaerobic, according to the metabolic processesthat take place. The metabolic equivalent (MET) represents the body’s energyconsumption during rest and it is used for quantifying fhisical activity (for example,a MET value of 3 would require 3 times the energy that is consumed at rest). Musclecontraction has two different phases: the isometric one (usually during the first partof the contraction) and the isotonic one. This article presents the interrelation ofphisical activity with with the complexity of metabolic patwais, bringing thearguments for the necessity of performing regular and controlled phisical activity.
Şerban Andrei Gâz Florea, Diana Ciurca, Anca Mare, Adrian Man, Bogdan Cordoş, Anda-Lavinia Grama and Daniela-Lucia Muntean
Background: Snake venom is a complex mixture of biologically active substances. Some peptides and proteins from snake have already demonstrated their therapeutically potential. The venom of Naja haje, an Elapidae member, has been analyzed from this point of view. Understanding the fully biochemical role of its enzymes has determined the scientists to find new separation and identification methods.
Objective: Our goal was to develop an optimal HPLC analytical method for separation and identification of Naja haje snake venom components, known for its neurotoxic activity. In addition, we wanted to find out if crude snake venom could inhibit the development of both Gram-positive and Gram-negative bacterial cultures. Materials and Method: Analysis of venom was performed on a HPLC system using a C16 column with UV detection at 210 nm. The analysis was done using two mobile phases, containing different concentrations of acetonitrile and trifluoroacetic acid aqueous solution at different pH values. An elution gradient was set at a flow of 0.60 mL/min. Bactericidal activity was quantified by measuring inhibition diameter around an aseptically disk filled with crude venom using Staphylococcus aureus and Escherichia coli. Results: An optimal HPLC analytical method has been developed by changing different parameters such as the pH value of mobile phase A or the elution gradient. The best resolution were obtained at a pH value of 7.4, in gradient varying from 5% to 45% in mobile phase B. Microbiological studies of the venom showed that Gram-positive bacteria growth was inhibited by crude venom, while on Gram-negative bacteria growth no effect was observed. Inhibition zone is dose-dependent and fresh crude venom is with 30% more potent than venom freeze and kept at -55°C. Conclusions: A comprehensive catalog of venom composition may serve as a starting point for studying structurefunction correlations of individual toxins for the development of new research tools and drugs of potential clinical use.
Adina Mitrea, Maria Mota, Daniela Patru, Amorin Remus Popa, Diana Protasiewicz, Robert Dinu, Cristina Muntean, Raluca Dina, Magda Sandu, Flavia Dinu and Betty Bornagel
As the latest data from the International Diabetes Federation (IDF) have shown,diabetes mellitus (DM) prevalence and incidence are continually increasing. Ourpurposes were to identify the socio-demographical characteristics of the subjectsdiagnosed with DM prior to this study, to establish the percent they represent of thestudied group, to evaluate the metabolic control of the subjects enrolled in the study,as well as to ascertain whether the blood glucose level or the glycated haemoglobin(HbA1c) measurement leads to the detection of a higher percentage of DM newlydiagnosed cases. The study conducted in 3 Romanian cities enrolled 4133 subjects(1340 from Craiova, 1224 from Oradea and 1569 from Sibiu). 1413 subjects (34%)reported having been diagnosed with DM prior to our study. Most of the subjectspreviously diagnosed with DM, 65%, take oral antidiabetic agents (OAA), 13% takeinsulin and 4% take both OAA and insulin. 20% of the subjects who did not reportDM had a blood glucose level ≥126mg/dL and 17% of them had an HbA1c ≥6.5%,leading to a high number of newly diagnosed DM cases, therefore emphasising theimportance of the simultaneous determination of these two parameters. The results ofthis study confirm the increasing prevalence of DM and the necessity of futurestudies in order to asses DM accurate prevalence in Romania.