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Hancu Gabriel, Tero-Vescan Amelia, Filip Cristina and Rusu Aura

Abstract

The aim of this study to inventory the main electrophoretic methods for identification and quantitative determination of fatty acids from different biological matrices. Critical analysis of electrophoretic methods reported in the literature show that the determination of polyunsaturated fatty acids can be made by: capillary zone electrophoresis, micellar electrokinetic chromatography and microemulsion electrokinetic chromatography using different detection systems such as ultraviolet diode array detection, laser induced fluorescence or mass – spectrometry. Capillary electrophoresis is a fast, low-cost technique used for polyunsaturated fatty acids analysis although their determination is mostly based on gas chromatography.

Open access

Ricuţa-Vasilica Dobrinoiu, Silvana Mihaela Dănăilă-Guidea, Rodica Ivan, Cristina Elena Filip and Fabio Massimo Sprio

Abstract

Paulownia ssp., in its places of origin, has been cultivated for over 2600 years and used as protection curtains against sand storms or floods, studies of how this species was exploited only fifty years ago in time what genetic research has only been affected in the last thirty years. The fundamental purpose of experimentation was to check the influence of different technological links (planting density and fertilization scheme), not only on the weight of the wood and the technological characteristics of the wood obtained, but also on the total biomass accumulation. The objectives of the research were: a) stems diameter increase, measured at 100 cm height from the soil surface, maintaining the harvest variant at 4 years; b) increasing of biomass accumulation. In all experimental variants, it is noted that the plants recorded higher values of the different variables analyzed (diameter at the base, normal diameter and total height), when the fertilization factor also intervenes. In the same way, when a higher fertilization was applied, higher values of the dendrometric indicators are obtained compared to the experimental variants that did not benefit from the supplementation of nutrients, suggesting that the most important risk factor in Paulownia plantations is the planting density and the fertilization element.

Open access

Amelia Tero-Vescan, Camil-Eugen Vari, Daniela-Lucia Muntean, Maria-Titica Dogaru, Cristina Filip and Silvia Imre

Abstract

A simple and selective method for genistein (GNST) determination in rat liver and kidney was validated in order to study the phytoestrogenic effect of GNST in ovariectomised female Wistar rats. GNST was separated on a Kromasil 100-RP8 column, 150 mm x 4.6 mm, 5 mm equipped with a Kromasil RP 8 precolumn. The mobile phase was 55:45 (v / v) phosphoric acid, 15 mmol in water: methanol at a flow rate of 1.3 ml / min. Luteolin 20 μg / ml in methanol was used as internal standard (IS). The retention time of GNST was tR = 13.22 min and tR =11.60 min for the IS. Calibration curves in the range 40-400 μg GNST/100g liver and 20-200 μg GNST/100g kidney presented a coefficient of determination higher than 0.99. The method developed presented a good precision and accuracy at the lower limit of quantification LLOQ. 10 white Wistar female rats, 8 weeks of age were treated s.c. with 10 mg GNST/kg bw/day for 8 weeks, while a group of 10 animals were used as controls. The values obtained for GNST in the liver were 192.12 ± 53.46 μg/100g and 74.51 ± 12.77 μg/100g in kidney samples.