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  • Author: Cosmin Matei x
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Abstract

The present paper describes the process of going through a pilot research phase, intuitive and scientific, from the desire to know what do we express in our corporality when we feel, empathize or play with the imaginary, especially in vocational area of actor’s training. This was done by working with the actor and Professor PhD András Hatházi, within a theatrical laboratory attended by the actor-students of the Hungarian Department, 2016-2019 promotion from the Babes-Bolyai University, Faculty of Theatre and Film, Cluj-Napoca. The objective of this research was given by the axiology emotion/feelings of emotion; heart/emotional system and brain/mind. Because the social, political, anthropological, and sentimental dimension of the human body has increased, so have the demands on the actors. As practitioners, we felt it necessary for the contemporary actor’s training to benefit from recent scientific observations about the bio-psycho-neuro-physiological processes of the living body, that is why the research has also evolved towards developing exercises to add new information to potentiate acting skills, at an imaginarycorporal level, as well as to achieve balanced parameters in terms of mental, emotional and physical health and integrity, especially post-acting.

Abstract

A rapid high performance liquid chromatography method, using a monolithic column, was developed for quantitative determinations of benzodiazepines (diazepam, clonazepam, lorazepam, midazolam) in whole blood. A liquid-liquid extraction step with n-chlorobutane isolates the drugs from alkalinized blood. The separation was carried out in reversed phase conditions using a Chromolith Performance (RP-18 100x4.6 mm) column. For the mobile phase, a mixture of a phosphate buffer (pH= 2.5)/acetonitrile (65/35 v/v), in isocratic mode at 2 mL/min. An ultraviolet spectrophotometer was used as the detector at the wavelength of 220 nm. The total run time of the analytical method is less than 4-6 minutes. The calibration curves showed linearity and the correlation coefficient of each individual curve was greater than 0.995. The method was linear over a concentration range of 0.03-0.6 μg/mL for clonazepam, lorazepam and midazolam. For diazepam of linearity was over the range 0.04-5.0μg/mL. Quantification limits ranged from 0.03-0.04μg/mL and the accuracy were from 80% to 105% for the recovery test.

The results indicate that this analytical method is simple, specific, accuracy, sensitive, demonstrating from the validation data and a higher robustness. The proposed method is applied routinely in forensic toxicological analysis involving blood.