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  • Author: Can Wei x
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Leiomyomatoid angiomatous neuroendocrine tumor of the myometrium

Abstract

Background: Only a few cases of Leiomyomatoid angiomatous neuroendocrine tumor (LANT) have been reported in the literature. Our case adds clinical, pathological, and immunohistochemical features.

Objective: To investigate the clinicopathological characteristics of LANT.

Material and Method: One case of LANT of the myometrium was reported in a review of literature. The morphological and immunohistochemical features were analyzed.

Results: In the course of an annual health examination of a 40-year-old woman, ultrasound results revealed a mass in the myometrium, which was clinically diagnosed as uterine leiomyoma. The patient underwent hysterectomy. Histological examination demonstrated that the tumor was composed of prominent vasculature and cellular stromal around vessels. Mitotic activity was absent. Both vascular and stromal cells showed diffusely expressed CD56 and chromogranin A. Stromal cells also expressed actin, SMA, and desmin, but not CK or HMB45. The pathological diagnosis was LANT of the myometrium. Follow up reported no evidence of recurrence three months after surgery.

Conclusion: LANT is a possible new disease entity. LANT is a dimorphic tumor consisting of smooth muscle and neurosecretory phenotype cells surrounding intratumoral vessels. Surgery may be the best treatment, resulting in good prognosis.

Open access
NPS2390, a selective calcium-sensing receptor antagonist controls the phenotypic modulation of hypoxic human pulmonary arterial smooth muscle cells by regulating autophagy

Abstract

Background and Objectives

Calcium-sensing receptor (CaSR) is known to regulate hypoxia-induced pulmonary hypertension (HPH) and vascular remodeling via the phenotypic modulation of pulmonary arterial smooth muscle cells (PASMCs) in small pulmonary arteries. Moreover, autophagy is an essential modulator of VSMC phenotype. But it is not clear whether CaSR can regulate autophagy involving the phenotypic modulation under hypoxia.

Methods

The viability of human PASMCs was detected by cell cycle and BrdU. The expressions of proliferation protein, phenotypic marker protein, and autophagy protein in human PASMCs were determined by western blot.

Results

Our results showed that hypoxia-induced autophagy was considerable at 24 h. The addition of NPS2390 decreased the expression of autophagy protein and synthetic phenotype marker protein osteopontin and increased the expression of contractile phenotype marker protein SMA-ɑ and calponin via suppressing downstream PI3K/Akt/mTOR signal pathways.

Conclusions

Our study demonstrates that treatment of NPS2390 was conducive to inhibit the proliferation and reverse phenotypic modulation of PASMCs by regulating autophagy levels.

Open access