Mirko Ivković, Jelena Stanivuk, Branko Jakovljević, Siniša Bjedov and Dušan Rajković
In a total mixed ration (TMR) feeding system, lambs, as well as other animals, tend to exhibit dietary preferences. The purpose of this paper is to examine differences in the feed selection behavior between lambs previously accustomed to TMR feeding and lambs newly introduced to TMR diets.
A total of eight male Suffolk lambs were allocated to two groups of four each. Both groups received the same feed formulation. The roughage portion of the diet consisted of alfalfa hay, whereas the concentrate portion included a concentrate mixture for finishing lambs. One group of lambs was fed total mixed rations containing 30% alfalfa hay and 70% pelleted concentrate. The other group received alfalfa hay and concentrate rations separately as single diets. Diet selection measurements were performed after four weeks of the experimental feeding regime.
The results obtained indicate a clear preference of both groups for concentrate (112±5% and 110±6% respectively) over alfalfa hay (72±11% and 77±14% respectively). Significant differences were recorded in the duration of feeding time. The lambs accustomed to TMR feeding consumed 400 g of TMR for 18±1 minutes, whereas the lambs newly introduced to TMR diets required 154±88 minutes for the same allotment. Moreover, the lambs starting on a TMR diet were found to consume feed markedly slower, although their diet selection proved equally successful compared to the lambs accustomed to TMR feeding.
Anđelo Beletić, Duško Mirković, Nebojša Antonijević, Valentina Đorđević, Violeta Šango, Branko Jakovljević, Jovan Peruničić, Mirka Ilić, Zorana Vasiljević and Nada Majkić-Singh
Incidence of Hyperhomocysteinemia and Mthfr C677T Polymorphism Among Young Patients with Acute Myocardial Infarction
Hyperhomocysteinemia is considered an independent risk factor for premature cardiovascular disease. Mutation MTHFR C677T reduces the activity of methylenetetra-hydrofolatereductase and may cause hyperhomocysteinemia. Incidence of hyperhomocysteinemia (homocysteine above 12 μmol/L), homocysteine level, and distribution of MTHFR C677T genotypes (C/C, C/T and T/T) are compared between young patients with acute myocardial infarction and healthy persons, matched by age. Study involved 86 patients younger than 45 years (77 men and 9 women) and 35 controls. Homocysteine was measured by an HPLC method and the MTHFR C677T genotype determined using PCR amplification and digestion with Hinf I. Statistical analyses included chisquare and Mann-Whitney U tests. Hyperhomocysteinemia was present in 32.6% patients and 14.3% controls, revealing a significant difference (P= 0.038). Median homocysteine levels in patients (10.4 μmol/L) and controls (9.6 μmol/L) were significantly different (P=0.035). Among patients, 50.0% had C/C, 41.9% C/T and 8.1% T/T genotype, and the genotype had no influence on hyperhomocysteinemia incidence and homocysteine level. Genotype distribution in patients was not significantly different from that observed in controls. The conclusion is that young patients with acute myocardial infarction have higher incidence of hyperhomocysteinemia and higher homocysteine levels than healthy young adults, while there is no significant difference in the distribution of MTHFR C677T genotypes.