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Open access

Marija Živković Sandić, Neda Stefanović, Branka Popović and Branislav Glišić


Tooth agenesis is common dentofacial malformation in humans. Its etiology is still not clear. Hypodontia has been regarded as a multifactorial condition influenced by gene function, environmental interaction and developmental timing. More than 300 genes have been related with patterning, morphogenesis and cell differentiation in teeth. According to data WNT10A gene is considered to have an important role in odontogonesis. The aim of this study was to show mutation status in WNT10A gene in a family with two members with diagnosis of hypodontia/oligodontia. In the reported family (father, mother, son, daughter) children were diagnosed with congenital tooth agenesis (son-2 teeth, daughter-11 teeth), while parents negated congenital absence of teeth. We identified a heterozygous missense mutation, c.682T>A (p.Phe228Ile) within the exon 3 of WNT10A in mother and father and the same homozygous mutation was detected in the same region of WNT10A gene in daughter and son. Observed differences in our study, from no symptoms to mild/severe hypodontia, could be the consequence of genetic influence of c.682T>A(p.Phe228Ile) mutation, but also the contribution of many environmental factors during odontogenesis.

Open access

Dijana Trišić, Bojana Ćetenović, Igor Jovanović, Elizabeta Gjorgievska, Branka Popović and Dejan Marković


Background/Aim: The aim of this in vitro study was to investigate the influence of irradiation cycles and resting periods, on thermal effects on the external root surface during root canal irradiation of two diode laser systems (940 nm and 975 nm), at output powers of 1 W and 2 W in continuous mode. In previous studies the rising of temperature above 7°C has been reported as biologically accepted to avoid periodontal damage on the external root surface. Material and Methods: Twenty human inferior incisors were randomly distributed into four groups, the 940 nm, and the 975 nm diode laser irradiation, both with an output power of 1 W and 2 W, in continuous mode. The thermographic camera was used to detect temperature variations on the external root surface. Digital radiography of the samples was made. Results: After three cycles of irradiation, at apical third of the root, mean temperature variation by 940 nm diode laser irradiation was 2.88°C for output power of 1 W, and 6.52°C for output power of 2 W. The 975 nm laser caused a higher temperature increase in the apical region, with temperature variation of 13.56°C by an output power of 1 W, and 30.60°C at 2 W, with a statistical significance of p ≤ 0.0001 between two laser systems compared for the same power. The resting periods of 20 s between cycles were enough to lower temperature under 7°C in the case of 1 W and 2 W for 940 nm diode laser, while for 975 nm laser, after three irradiation cycles overheating occurred at both output power rates. Conclusion: Three cycles irradiation of 940 nm diode laser, with resting periods of 20 seconds, allowed safe usage of 1 W and 2 W in CW for endodontic treatment. For 975 nm at a power rate of 1 W, the last resting period drop the temperature near the safe limit and it came under 7°C in a period less than a minute, while at the power of 2 W the resting periods were not long enough for the safe temperature decrease.

Open access

Gavrilo Brajović, Nataša Nikolić-Jakoba, Branka Popović, Vesna Ilić, Sonja Mojsilović, Dragana Marković and Nadežda Milošević-Jovčić


Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls.

Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules.

Results IgG content in GCF was five times higher in patients with moderate (p<0.01) and eight time higher in patients with advanced periodontitis (p<0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p<0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules.

Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects

Open access

Jelena Milovanovic, Aleksandar Arsenijevic, Bojana Stojanovic, Marija Milovanovic, Stipan Jonjic, Branka Popovic, Nebojsa Arsenijevic and Miodrag L. Lukic


Viral infection has been identified as the most likely environmental trigger of multiple sclerosis (MS). There are conflicting data regarding the role of cytomegalovirus (CMV) in MS pathogenesis.

We utilised experimental autoimmune encephalomyelitis (EAE)-resistant BALB/c mice and murine cytomegalovirus (MCMV), the murine homolog of CMV, to examine the mechanism by which viral infection enhances autoimmune neuroinflammation. Mice subjected to latent neonatal MCMV infection developed the typical characteristics of EAE. Similar to MS, the MCMV-infected EAE-induced mice developed infiltrates in the central nervous system (CNS) composed of similar percentages of CD4+ and CD8+ T cells. The influx of both Th 1 and Th 17 cells into the CNS of MCMV- infected EAE-induced mice was observed. Interestingly, the development of autoimmune neuroinflammation after latent MCMV infection was accompanied by a significant influx of Tc17 cells (CD8+IL-17+ and CD8+RoRγt+) but not Tc1, cells. Our results suggest that latent MCMV infection affects the development of inflammatory lymphocytes that exhibit encephalitogenic potential, thereby mediating increased CNS pathology following EAE induction, and that CMV represents a possible environmental factor in the pathogenesis of MS and other autoimmune diseases

Open access

Zorica Vasiljević, Nikola Popović, Bojan Dimitrijević, Dragan Vujović and Branka Kalanović Bulatović


Viticulture is an important branch of Serbian agriculture. It has a long tradition, but there is the decreasing trend both in areas under vineyards and in grape production. The analysis intends to answer the question what are the reasons for those trends. The main goal of the paper is to analyse the relationship between the decrease in areas under vineyards and the level of investment effectiveness in this type of production. The paper analyses the influence of the governmental agrarian policy measures onto increase of investments′ effectiveness in vineyard establishment. The government has adopted the measures for subsidising the establishment of vineyards. The research analyses the economic effectiveness of investments by using the calculation of investments as well as dynamic multi-periodic methods of investment analysis (Net Present Value - NPV and Internal Rate of Return - IRR). The data for investment analysis (investments, the value of production, operating costs) have been collected by the farms through the survey made in Central Serbia, while the data on the governmental subsidies have been taken from The Ministry of Agriculture, Forestry and Water Management of the Republic of Serbia. The analysis has shown that investments into vineyards are not profitable without governmental subsidies and support.