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  • Author: Bozidar Savic x
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SAVIĆ Božidar, RADANOVIĆ Oliver, JOVIČIĆ Dubravka, NEŠIĆ Ksenija, IVANOVIĆ Snežana, STEVANČEVIĆ Ognjen, CVETOJEVIĆ Đorđe and KASAGIĆ Dragan

Abstract

A retrospective study on 235 natural cases of Porcine Respiratory Disease Complex in order to determine the etiological agents, their prevalence and interrelationships was performed in Serbia. Lung tissue samples were analyzed by Polymerase Chain Reaction for the presence of Porcine circovirus type 2, Porcine reproductive and respiratory virus, Swine influenza virus, Mycoplasma hyopneumoniae, Pasteurella multocida, Actinobacillus pleuropneumoniae, Haemophilus parasuis, Streptococcus suis and Arcanobacterium pyogenes. A total of 49 different combinations of viral and bacterial pathogens were found. Five different viral and viral/Mhp co-infections were detected. Monobacterial infections were found in 150 cases and polybacterial infection was detected in 85 samples. PCV2 was the main virus detected, and Pm was the most aggressive secondary pathogen detected in PRDC. The reason for PRDC being so prevalent among Serbian pigs is most likely due to the large number of risk factors in the conventional farrow-to-finish system, compared to multi-site production systems. Therefore, measures aimed at a better control of respiratory viruses, particularly Porcine circovirus type 2 and Porcine reproductive and respiratory virus, as well as Mycoplasma hyopneumoniae infections, and adoption of rational decisions on respiratory bacterial pathogens specific therapeutic and preventive strategies at herd level, simultaneously with significant improvements on farm management should reduce the occurrence of PRDC.

Open access

Oliver Radanovic, Jadranka Zutic, Dobrila Jakic-Dimic, Branislav Kureljusic and Bozidar Savic

Abstract

The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37°C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.

Open access

Igor Stojanov, Ivan Pavlović, Ivan Pušić, Jasna Prodanov-Radulović, Radomir Ratajac, Doroteja Marčić and Božidar Savić

Abstract

The aim of our study was to determine whether and to what extent certain species of helminths and protozoa are present in the wild boar population living in hunting grounds in Vojvodina. For this purpose, 52 faecal samples of hunted wild boars (aged 6 months to 2 years) were examined. Examination of the faeces was performed using classical coproscopic laboratory methods. The following parasite species were identified: Metastrongylus spp. Ascaris suum, Trichuris suis, Physocephalus sexalatus, Strongyloides ransomi, Oesophagostomum sp. / Globocephalus sp., Hyostrongylus rubidus, Gnathostoma hispidum, Eimeria deblecki and Eimeria suis. The obtained results from this study indicated that wild boars are a potential reservoir of a variety of endoparasites, thus endangering the surrounding ecosystem.

Open access

Delić Nikola, Drašković Vladimir, Stevanović Jevrosima, Savić Božidar, Lakić Nada, Bošnjak-Neumüller Jasna and Stanimirović Zoran

Abstract

Control of swine dysentery with antibiotics is often ineffective due to the resistance of Brachyspira hyodysenteriae. The potential of some herbal-based components against B. hyodysenteriae was previously studied in vitro. This study aims at the evaluation of in vivo efficacy of phytogenic feed additives in the control of swine dysentery

The study involved 64 seven-week old weaned pigs allotted to 4 groups: two were fed on feed supplemented with either Patente Herba® or Patente Herba® Plus, the third received tiamulin (positive control), while the negative control was not given antibiotics or additives. Fecal consistency was recorded daily. The presence of B. hyodysenteriae in the feces was investigated weekly using microbiological assays and the PCR test. Weight gain and feed conversion ratio were calculated for each week, and for the whole experiment.

B. hyodysenteriae was detected in all samples by both methods. The additives showed efficacy in the prevention and control of swine dysentery as only normal and soft stool was observed in the treated groups. By contrast, in the negative control all feces categories were detected. Frequencies of feces categories significantly differed (p<0.001) between feed-supplemented groups and the negative control. Efficacy of both additives in the prevention of SD is comparable to tiamulin, based on insignificant differences in the frequency of the various feces categories.

Beneficial effects of both additives resulted in significantly (p≤0.05) higher weight gain and lower feed conversion ratio in comparison to the negative control. The average weight gains between additive-fed groups and tiamulin-treated group did not differ significantly.