Introduction: The inflorescence of Helichrysum arenarium (L.) Moench shows anti-inflammatory, antioxidant, detoxifying properties and is traditionally used in liver and biliary tract diseases. Because of its difficult and expensive cultivation, the plant raw material is mainly harvested from natural sites.
Objective: The research aimed to determine the local variation in yield and content of flavonoids and phenolic acids in the yellow everlasting inflorescence against the background of the layer structure of vegetation as the rate of plant succession.
Methods: The plant raw material was collected from 30 plots of 1 m2, established for three separate populations developing on sandy fallows near Zielona Góra (western Poland). For each study area, percentage cover of the moss-lichen and herb layers, the height, cover and yield of H. arenarium as well as the height and cover of other herbaceous plants were determined. Total contents of flavonoids (expressed as quercetin) and phenolic acids (calculated as caffeic acid) were measured spectrophotometrically, according to Polish Pharmacopoeia.
Results: Everlastings reached a cover of up to 70% and the maximum air-dry matter yield of 46.42 g/m2. The height, coverage and yield of H. arenarium were correlated with the parameters describing the herb layer. The content of flavonoids ranged from 0.56 to 0.99%, while that of phenolic acids from 0.82 to 1.80% DM.
Conclusions: Yellow everlasting is an important species of early fallows on poor sandy soils and these habitats constitute a rich natural source of herbal raw material. Inflorescences harvested from natural sites are distinguished by a high and similar content of polyphenols and usually meet the requirements of Polish Pharmacopoeia.
In our research, the concentration of lotaustralin in the roots of two species Rhodiola kirilowii and Rhodiola rosea were compared. Aqueous and hydroalcoholic extracts from those plants were analyzed too. To determine the content of this compound the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. The obtained results showed that the content of measured lotaustralin depends on the species of Rhodiola. R. rosea roots are the richer source of lotaustralin then R. kirilowii. The same situation was observed in the extracts. A hydroalcoholic extract from R. rosea contains up to 135.276 mg of lotaustralin in 100 g of dry powdered material. In the case of R. kirilowii extracts, an aqueous extract contained more lotaustralin (74.791 mg/100 g of dry powdered material) then a hydroalcoholic extract.
The aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.
The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.