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Biljana Poposka, Snezhana Smickova, Simonida Crvenkovska, Beti Ivanovska, Tome Stefanovski, Marija Metodieva and Gordana Petrusevska

Prognostic Value of the Expression of p53 in Patients with Non-small Cell Lung Cancer

Background. The p53 gene is frequently mutated in non-small-cell lung cancer (NSCLC). However, the effect of p53 gene mutations on patient prognosis remains unclear.

Aim. The aim of this study is to determine the association of p53 abnormalities with clinical data and prognosis in patients with NSCLC.

Material and Methods. Tumor tissues from 80 patients with NSCLC were assessed by immunohistochemistry for expression of p53. The immunohistochemical study was performed on formalin-fixed, paraffin-embedded sections using LCAB immunoperoxidase method with mouse anti-p53 monoclonal antibody (clone DO-7; DAKO).

Results. Forty-three (53,75%) of 80 patients revealed aberrant immunostaining for p53. Except to the histological type of tumors, there was no correlation of p53 expression with the clinicopathologic features. The Kaplan-Meier survival analysis demonstrated that patients with p53 positive status had poor survival rate (p=0,005 by the Log Rank test= 7,914). In Cox, regression analysis p53 and performance status emerged as independent prognostic factors (p<0,009 and p<0,000 respectively).

Conclusion. The results from this study indicated that the aberrant expression of p53 is significant and independent predictable prognostic factor for patients with NSCLC.

Open access

Biljana Poposka, Snezana Smickova, Simonida Crvenkovska, Beti Ivanovska, Tome Stefanovski and Gordana Petrusevska

Prognostic value of immunohistochemical expression of HER-2/neu in patients with lung carcinoma

Background. The amplification and the overexpression of the Her-2/neu gene have been shown in certain human tumours and are postulated to be important in human carcinogenesis. In this study we evaluated the expression of HER-2/neu gene in patients with lung carcinoma (LC) and assessed its prognostic significance.

Patients and methods. HER-2/neu expression was determined in 127 LC patients using immunohistochemistry (IHC) performed on paraffin-embedded section - Hercep Test™ (DAKO).

Results. The overall HER-2/neu expression was seen in 36 (28.35%) of 127 LC patients. According to the histological type, HER-2/neu overexpression was detected in 12 patients with adenocarcinomas (60%), in 19 patients with squamous cell carcinomas (31.14%), in 4 patients with small cell-lung carcinomas (10%) and in 1 patient with other carcinomas (16.66%). Only in patients with small cell-lung carcinomas HER-2/neu overexpression was in correlation with the stage of the disease (p<0.001). The patients with HER-2/neu positive expression were associated with a significantly shorter survival compared with those who were HER-2/neu negative (log rank, p<0.002).

Conclusions. These observations suggest that HER-2/neu positivity may serve as a prognostic indicator in patients with LC. HER-2/neu plays a role in identifying patients at risk for the shortened survival, who may benefit from a more aggressive therapy.

Open access

Stanimir Dimitrov, Vesna Karapetkovska-Hristova, Ljupce Kochoski, Biljana Trajkovska, Borche Makarijoski, Vesna Prodanovska-Poposka and Godswill Ntsomboh-Ntsefong


The aim of this study was to analyze the effect of season and number of parity on the reproductive performance of sows kept on a commercial farm. To achieve this objective, 800 nrs productive parent sows and a total of 958 parities were analyzed. The results show that sow’s breeding season had a significant effect on the weaning-to-oestrus interval, the farrowing rate, the litter size and weaning piglets with positive results during autumn, winter and spring seasons. The number of parity has a significant effect on the weaning-to-oestrus interval, litter size and weaning piglets with acceptable values in sows with more farrowing. The results of this study on a pig farm with analysis which highlights the risk factors associated with reproductive performance of sows, could be used to improve sow farm practices.