The changes of the concentration of Cu and Zn in the soil solution and the percentage of particular forms of these elements in the soil solution were investigated in the long-term fertilization experiment. The soil solution was obtained following the vacuum displacement method. Speciation of copper and zinc ions was determined with MINTEQA2 for Windows software. The results of the investigation indicated that exclusive mineral fertilization (NPK) caused an increase of Cu and Zn concentration in the soil solution. Organic fertilization (FYM) resulted in a decrease of Cu and an increase of Zn concentration in the soil solution. Liming limited mobility of both analysed elements. The results of speciation analysis showed that regardless of the fertilization mode, the organo-mineral complexes are the main form of Cu occurring in soil solution. The percentage of Cu-DOC complexes ranges from 76.5 to 85.2% of the total concentration of Cu in the soil solution. The particular forms of copper can be sorted depending on the percentage in the soil solution as follows: Cu-DOC>Cu2+>Cu-CO3. The main form of Zn in the soil solution are active Zn2+ ions. The share of Zn2+ in total zinc concentration in the soil solution ranged from 76.9% to 86.4%. Forms of zinc in the soil solution can be arranged with regard to their percentage as follows: Zn2+>Zn-DOC>ZnCl+>ZnHCO3 +.