The objective of this study was to determine the basic physical and chemical parameters and the total content of Fe and Ni as well as their proportion in the fractions sequenced with Bureau of Reference (BCR) procedure in the selected organic materials used as soil fertilisers: bovine manure and chicken manure from broilers and layers. The highest content of dry matter, organic carbon and total nitrogen was found in the chicken layer manure. All examined materials had a comparable, C:N ratio (10–11:1). The highest amount of iron and nickel was found in the chicken broiler manure, whereas the lowest was in the chicken layer manure. The distribution of tested metals in the fractions sequenced with BCR procedure was differentiated. In the bovine manure and chicken broiler manure, the highest amount of iron was sequenced in the residual fraction, whereas in the chicken layer manure it was in the oxidative fraction. The oxidative fraction constituted the highest proportion in the total nickel content in the bovine manure and chicken layer manure, whereas in the chicken broiler manure it was the reducible fraction.