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  • Author: Augustin Curticăpean x
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Manuela Curticăpean and Augustin Curticăpean

Abstract

Since the release of the first genetically modified crop, scientists have pro and con opinion on cultivation and their use, because of potential health, and environmental risks. Current EU legislation (Directive 2003/18/EC) provides for public information, monitoring long-term effects, labelling and traceability at all stages of the placing on the market of GMOs. The aim of this study was to evaluate the quality of the food products on the market, in which the detection of presence / absence of GMO. In this regard, two types of maize flour and four types of soy products were analysed during 2013. The kit used for detection of the presence / absence of GMO in the samples tested, comprising the step of DNA extraction, DNA amplification by PCR and agarose gel electrophoresis of amplified products and uses two GM associated sequences - promoter 35S and NOS terminator from Agrobacterium tumefaciens. The presence of GMO was found in a corn sample, also in a soy sample the results illustrate the need for further analysis to identify the exact type and quantity of GMO (the limiting value imposed by European legislation being 0.9% at ingredient).

Open access

Lavinia Grama, Adrian Man, Daniela-Lucia Muntean, Șerban Andrei Gâz Florea, Francisc Boda and Augustin Curticăpean

Abstract

Polyoxometalates are important inorganic compounds with a broad range of pharmacological properties, including antiviral, antibacterial, antiprotozoal or antitumoral activities, even that their molecular mechanism of action is poorly understood. Purpose: In this paper we evaluated the antibacterial activity of some saturated polyoxotungstates (POW) compounds, since nowadays, the increasing resistance of bacteria to drugs represents a major health problem. Materials and methods: The antibacterial activity was studied by disk diffusion method as a possible screening method and by successive micro-dilutions method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been calculated for each compound by successive dilutions. We also compared the reliability of each testing method for this particular POW evaluation. Results: The best antibacterial activity was expressed by H4[SiW12O40]*nH2O and the lowest by Na3[PW12O40]*nH2O, but with very good activity on Staphylococcus spp., especially on MRSA. The POW activity occurs only at relatively high concentrations, and it is dependent on bacterial strain, with very good activity on Staphylococcus spp. The most reliable method for assessing the antibacterial effects of POW is micro-dilutions. POWs could be practically applied in hospital decontamination and could have a possible in vivo antibacterial application.

Open access

Lavinia Berta, Andrei Gâz, Francisc Boda and Augustin Curticapean

Abstract

Objective: The main objective of this research is to find the coordination ratio between AsW9 and Ag+, as a preliminary study for synthesizing a new silver-arsenotungstate complex. Material and method: The ligand:cation molar ratio in complexes was determined by conductometric and potentiometric titrations of AsW9 with silver salts: CH3COOAg, AgNO3. Results: The ratio was obtained from the inflexion points of the curves when molar ratio was plotted versus conductivity, or from the equivalence point when silver added volume was plotted versus pH value. Each graphic shows one point of inflexion corresponding to 1:1.54 ratio of AsW9:Ag+. In the same manner, the equivalent volumes determined by graphical method gave the ratio 1:1.53. The spectral results confirmed that a AsW9:Ag+ complex was formed since the ligand absorption maxima values have been changed from 190 nm to 197 nm in the case of using AgNO3 and 196 nm for CH3COOAg corresponding to the W=Od bond, and from 246.5 nm to 274 nm (AgNO3) and 270 nm (CH3COO-Ag+) for the W-Ob,c-W bond. Conclusions: Silver cation exhibit a preference for AsW9 in a ratio of 3 to 2. This ratio can be associated to a sandwich type arrangement, with two trilacunary Keggin building blocks incorporating 3 metal cations in a tetrahedral geometry.