The influence of methyl jasmonate on anthocyanin accumulation in roots of Kalanchoe blossfediana plants was studied. Methyl jasmonate (JA-Me), at a concentration of 5.0 to 40.0 mg.l−1, substantially increased anthocyanin accumulation in roots of intact plants, when it was applied as a solution under natural light conditions. The production of anthocyanin depended on the concentration of methyl jasmonate and the age of the plant. The stimulatory effect was higher in older plants of K. blossfeldiana than in younger ones. When leaves were removed methyl jasmonate slightly stimulated anthocyanin accumulation compared with intact plants. The obtained results indicate that leaves are necessary for the anthocyanin accumulation in the roots. In isolated roots methyl jasmonate did not affect the accumulation of anthocyanins in light conditions. Seven anthocyanins were documented in the roots of control plants and 8 anthocyanins in the roots of JA-Me treated ones. JA-Me increased the level of anthocyanins in roots of old K. blossfeldiana plants 6.8, 6.0 and 3.6-folds, after 4, 8 and 14-days of treatment, respectively.