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Open access

Artur Stopyra and Anna Snarska

Abstract

The aim of the study was to determine the suitability of basic haematological, biochemical, and gasometric tests in checking the effectiveness of transfusion therapy in foals during isoerythrolysis. The number of red blood cells, haemoglobin, haematocrit, and partial pressure of carbon dioxide, oxygen, and blood pH was determined immediately before and several times after blood transfusion. The concentration of serum free bilirubin was also measured to confirm haemolysis. Fluids (0.9% NaCl, multielectrolytic fluid, 5% glucose) and antibiotics (penicillin, amikacin) were provided to the foals. The lowest values of haematological parameters were observed before transfusion. This was accompanied by decreased partial pressure of oxygen, low pH, and increased arterial carbon dioxide tension. Transfusion of whole blood led to a gradual normalisation of the haematological parameters, also accompanied by the normalisation of gasometric indicators (decrease in pCO2 and pO2 and pH increase). Monitoring of selected haematological and gasometric parameters allows to evaluate the efficacy of blood transfusion during treatment of haemolytic disease of foals.

Open access

A. Snarska and P. Sobiech

Abstract

The aim of the study was to evaluate the megakaryocyte lineage of bone marrow and coagulation parameters in fallow deer during the last month of pregnancy. The animals were managed in the barn-feeding system. Twenty female fallow deer, aged 2-3 years, divided into 2 groups were used in the study. Group 1 comprised the females in the last month of pregnancy, and the non-pregnant females were used as the control. All the animals were clinically healthy. Coagulation parameters were measured in all the deer: thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), and plasma concentrations of fibrinogen, D-dimer, and antithrombin III. A quantitative assessment of bone marrow was carried out for the erythroblastic, myeloid, lymphoid, monocyte-macrophage, and megakaryopoietic cell lines. A detailed analysis of megakaryocyte lineage was performed after whole blood and platelet count.

There were no significant differences in the erythroblast, granulocyte, monocyte-macrophage and lymphoid systems between the animal groups. Thrombocyte count in the pregnant deer was lower than that found in the control group. Bone marrow smears revealed a slightly decreased megakaryocyte count, while the megakaryoblast and promegakaryocyte counts were unchanged. The analysis of coagulation parameters showed increased levels of fibrinogen, thrombin time, prothrombin time and activated partial thromboplastin time in the pregnant animals. The study suggested a hyperactivation of the coagulation system with a slight reduction in the megakaryocyte count in bone marrow, and a reduction in platelet count in peripheral blood at the end of pregnancy.

Open access

Anna Snarska, Andrzej Pomianowski, Wioletta Krystkiewicz, Przemysław Sobiech, Sylwia Lew and Dariusz Bednarek

Abstract

Blood morphology in dogs infected with intracellular parasites of the genera Babesia and Ehrlichia was examined. The parasites were detected in peripheral blood smears stained by the May-Grünwald-Giemsa method. During the parasitic invasion, a decrease in thrombocyte counts was observed. This was connected with forming platelet aggregates. It was noted that the number of giant platelets simultaneously increased.

Open access

Anna Snarska, Dominika Wysocka, Liliana Rytel, Krystyna Makowska and Sławomir Gonkowski

Abstract

Introduction: Bisphenol A (BPA) is a substance widely used in industry for the production of polycarbonates and epoxy resins used in packaging and containers for beverages, contact lenses, compact discs (CDs), window panes, and many other elements. This compound belongs to the group of polyphenols and xenoestrogens commonly found in the human environment. What we know about BPA is still insufficient to enable us to protect our health against its adverse effects, and current knowledge of the influence of BPA on erythroblastic cell lines in bone marrow is rather fragmentary. The aim of the experiment was to assess the effect of two doses of BPA (0.05 mg/kg and 0.5 mg/kg b.w. per day) on myeloid haematopoiesis.

Material and Methods: During this experiment, the number of all types of cells in the erythroblastic cell line was evaluated in porcine bone marrow before and after BPA administration.

Results: The obtained results clearly indicate changes in haematopoietic activity of the bone marrow, which was demonstrated by a decrease in erythroblastic cell line production in both experimental groups. The haematological effects of the bone marrow changes were anaemia, caused by a number of erythrocytes which was depressed due to their immaturity, and a significant decrease in mean cellular volume in both groups.

Conclusion: The harmful effect of high and low doses of BPA on haematopoietic processes was proved.

Open access

Anna Witt-Majchrzak, Piotr Żelazny and Jadwiga Snarska

Abstract

Hospital infections, and in particular infections of the surgical site are a common problem of the procedural departments. Due to continuous progress of surgical techniques and patient population getting older with multiple co-morbidities, multidirectional actions need to be taken to avoid these infections or, if they do occur, achieve optimal treatment outcomes. Vacuum wound therapy is one of the directions that has been developed over the recent years.

The aim of the study evaluate wound healing in patients after an off-pump coronary artery bypass grafting procedure, using the internal mammary artery, treated with negative pressure wound therapy system.

Material and methods. This prospective, open label study evaluated healing of postoperative sternotomy wounds after their primary closure with negative pressure wound therapy, using continuous negative pressure of -80 mmHg in 40 patients and 40 patients in a control group in whom conventional dressings were applied in the postoperative period.

Results. The number of patients in whom primary wound healing occurred without complications was significantly higher in the negative pressure wound therapy group versus the control group (x2 test =4.50, p=0.0339) and the number of total superficial infections was significantly smaller versus the control group (x2 test =5; p=0.0254). Antibiotic therapy was also initiated significantly less often as compared to the group treated with conventional dressings (x2 test = 4.11; p=0.0425).

Conclusions. Negative pressure wound therapy after primary wound closure reduces the risk of superficial infections in the population with multiple risk factors of complications in the sternotomy wound healing.

Open access

Anna Snarska, Dominika Wysocka, Liliana Rytel, Sławomir Gonkowski, Hanna Pawelec and Przemysław Sobiech

Abstract

Introduction: Simvastatin is a substance which is commonly used as a medicine to reduce cholesterol level. Unfortunately, it shows numerous side effects. Simvastatin affects various internal organs, and among other detriments to health may cause persistent muscle weakness, osteolytic processes, headaches, and rashes. Until now knowledge of the influence of simvastatin on bone marrow cells has been rather scant and fragmentary.

Material and Methods: During this experiment the numbers of all types of cells in the leukocytic system of porcine bone marrow were evaluated after 28 and 56 days of oral administration of simvastatin at a dose of 40 mg/day/animal.

Results: Simvastatin caused an increase in the number of all types of cells in the leukocytic system, and the most visible fluctuations concerned promyelocytes.

Conclusion: Observations obtained during the present study indicated that the results of the action of simvastatin on porcine bone marrow differ from those observed in other mammal species, including human. This may be due to various metabolic pathways within the bone marrow in the particular species, but the exact mechanisms of these actions are unknown at the present time.