Search Results

You are looking at 1 - 2 of 2 items for

  • Author: Angelika Voronova x
Clear All Modify Search
Open access

Angelika Voronova and Dainis Ruņģis

Abstract

Conifer genomes are large and stably diploid, in contrast to angiosperms, which are more variable both in genome size and ploidy. Conifer genomes are characterised by multiple gene families and pseudogenes, contain large inter-gene regions and a considerable proportion of repetitive sequences. All members of plant retrotransposon orders have been identified in gymnosperm genomes, however active elements have not been described. Investigation of transposable elements in Scots pine (Pinus sylvestris L.) could offer insights into transposon-mediated reorganisation under stress conditions in complex and ancient plant genomes. Nine Pinus sylvestris specific markers were developed to hypothetical long terminal repeats (LTRs) from differentially expressed retrotransposon-like fragments after heat stress and insect damage. Genetic diversity of 150 trees from a naturally regenerated pine stand was investigated using the IRAP method. The developed markers revealed high levels of genetic diversity and were able to distinguish subpopulations growing in long-term differential environmental conditions. Somaclonal variation was also investigated using these markers and polymorphic fragments were identified between ramets of Scots pine clones growing in two different plantations, possibly indicating evidence of recent transposition events. Sequencing of the polymorphic fragments identified two groups of sequences containing LTR sequences of an unknown retrotransposon with homology to the LTRs of the Copia-17-PAb-I element.

Open access

Agnese Gailīte, Anita Gaile, Ilze Gaile, Angelika Voronova, Ilze Veinberga, Aina Kokare and Dainis Edgars Ruņģis

Rye (Secale cereale L.) is an important grain crop in Latvia, where it is mainly used for baking rye bread, which is a popular staple. However, the area under rye cultivation in Latvia is small, and the majority of varieties planted are foreign. In 1937, almost 290 000 ha of rye were planted, while in 2011, only 28 000 ha were planted, or ~5% of the area planted with cereals. The Latvian rye genetic resources collection contains nine accessions, including old and new cultivars, landraces and one repatriated accession, which was previously held in the N. I. Vavilov Research Institute of Plant Industry collection. A set of descriptors has been developed for rye, and field evaluations of the Latvian rye collection have been started. A set of simple sequence repeat (SSR) markers has been utilised for genetic fingerprinting of the collection. The initial genetic results indicate that the Latvian rye collection contains a high degree of genetic diversity. Analyses are continuing in order to more fully characterise the collection both phenotypically and genetically.