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Sergiu Georgescu, Oana Canareica, Andreea Dudu and Marieta Costache

ABSTRACT

Sturgeons such as Acipenser baerii and Acipenser gueldenstaedtii are the most common species farm raised worldwide in aquaculture, because of the dwindling natural sources of caviar and meat. Also, these species can easily participate in the formation of an intraspecific hybrid with a great potential for growth in aquaculture.

Microsatellites are nuclear markers consisting of short repetitive sequence, dispersed across the entire genome with characteristics such as relatively small size and high level of polymorphism. The aims of the present study were to optimize a protocol for microsatellite multiplexing and analysis of genetic diversity in hybrid sturgeons farmed in Romania.

Genomic DNA was isolated from fins, and four pairs of primers were designed to amplify microsatellite loci: LS 19, LS 68, Aox 9, and Aox 45. Amplification of the microsatellite loci was carried out in one 3-Plex reaction for LS 19, LS 68, and Aox 9, and monoplex reaction for Aox 45. For an individual locus we obtained four alleles for Aox 45, eleven alleles for Aox 9, six alleles for LS 68, and eight alleles for LS 19. The results will be applied to test the broodstocks at Romanian hatcheries and to increase the efficiency of breeding.

Open access

Alexandru Burcea, Iulia Elena Florescu, Andreea Dudu, Sergiu Emil Georgescu and Marieta Costache

Abstract

Due to construction of the Iron Gates dams, the Lower Danube has suffered a decrease in sturgeon populations. The dams have decreased sturgeon habitat area, which in turn has caused an overlap of reproduction areas for all sturgeon species. The ease with which sturgeon species can create hybrid offsprings gave rise to an increase in the number of hybrid sturgeon species now found in the Lower Danube area. We propose a set of molecular methods for hybrid species using DNA markers represented by microsatellites and mitochondrial DNA. This identification data and methodology is important for use on sturgeon farms due to the need to correctly identify species of sturgeons. Using the proposed methodologies, it is possible to avoid identification errors that might appear when using only morphological criteria to idenfy sturgeons

Open access

Gina-Oana Popa, Miad Khalaf, Andreea Dudu, Angela Curtean-Bănăduc, Doru Bănăduc, Sergiu Georgescu and Marieta Costache

ABSTRACT

Among the species of salmonids that exist in Romania, the most common is the brown trout (Salmo trutta, morpha fario, Linnaeus, 1758), with a high commercial potential, being used for aquaculture or fishing. Unfortunately, its natural habitat is disrupted by human activities and in order to avoid local extinction of Salmo trutta fario repopulation strategies are being applied. The repopulation activities must be carefully designed and conducted taking into consideration that there is a high risk of hybridization. Our study aims to analyze the genetic diversity of three Romanian brown trout populations found in rivers in the Făgăraş Mountains using mitochondrial markers.

Standard DNA extraction protocol with phenol-chloroform was applied to the biological material represented by small fin fragments sampled from 80 individuals of brown trout, followed by PCR amplification of D-loop control region and Sanger sequencing. Alignment and editing of all the sequences obtained were carried out with Bioedit, the phylogenetic tree construction was performed by Neighbour Joining method implemented in MEGA v5, and intra- and interpopulational diversity was evaluated with DNAsp v5 and MEGA v5.

For the three brown trout populations, 13 haplotypes were identified for N1, 11 for N2 and 10 for N4, with a haplotype diversity greater than 0.8. The phylogenetic tree topology showed that individuals chosen for this study were placed in the Danubian clade as the other Danubian sequences selected from GenBank.

The study proved that the three Salmo trutta fario populations analyzed were genetically distinct and that in Romania there are still are pure Danubian brown trout populations.