Vladimir Bacârea, Petruş Bacârea and Anca Bacârea
Dana Bădău, Anca Bacârea, Ramona Natalia Ungur, Adela Bădău and Alina Mirela Martoma
Objective: The aim of our research was to identify physiological and biochemical changes induced by training at medium altitude.
Methods: Ten biathlon athletes underwent 28-day training camp at medium altitude in order to improve their aerobic effort, following the living high-base train high-interval train low (Hi-Hi-Lo) protocol. There were investigated three categories of functional and biochemical parameters, targeting the hematological changes (RBC, HCT, HGB), the oxidative (lipoperoxid, free malondialdehyde and total malondialdehyde) and antioxidative balance (the hydrogen donor capacity, ceruloplasmin and uric acid) and the capacity of effort (the maximum aerobic power, the cardiovascular economy in effort, the maximum O2 consumption).
Results: All the biochemical and functional evaluated parameters showed significant increases between the pre-training testing and post-training testing (5.13 ± 0.11 vs. 6.50 ± 0.09, p < 0.0001 for RBC; 44.80 ± 1.22 vs. 51.31 ± 2.31, p < 0.0001 for HCT; 15.06 ± 0.33 vs. 17.14 ± 0.25, p < 0.0001 for HGB; 1.32 ± 0.04 vs.1.62 ± 0.01, p < 0.0001 for LPx; 1.61 ± 0.01 vs. 1.73 ± 0.01, p < 0.0001 for free MDA; 2.98 ± 0.08 vs. 3.37 ± 0.03, p < 0.0001 for total MDA; 45.92 ± 0.13 vs. 57.98 ± 0.12, p < 0.0001 for HD; 25.95 ± 0.13 vs. 31.04 ± 0.06, p < 0.0001 for Crp; 3.47 ± 0.03 vs.7.69 ± 0.02, p < 0.0001 for UA; 63.91 ± 1.00 vs. 81.53 ± 1.97, p < 0.0001 for MAP; 33.13 ± 0.57 vs. 57.41 ± 0.63, p < 0.0001 for CVEE; 4190 ± 50.45 vs. 5945 ± 46.48, p < 0.0001 for VO2max).
Conclusions: Aerobic effort capacity of biathlon athletes has increased in the post-training period, using Hi-Hi-Lo protocol.
Nicoleta Dora Pop, Anca Bacârea, Ligia Coroș, Grigore Aloiziu Dogaru, Ioan Hosu, Vladimir Bacârea and Attila Nagy
In this study, different aspects of anemia in chronic kidney disease have been observed, starting from the fact that the severity of anemia is associated with the degree of kidney dysfunction, the main cause being the erythropoietin deficiency, which is synthesized mostly by the kidneys. 58 persons were included in this study, 19 patients with non-dialysis-dependent chronic kidney disease, 18 patients with chronic kidney disease who received kidney transplantation and 21 apparently healthy persons. We evaluated the serum level of erythropoietin, serum creatinine, proteinuria, the glomerular filtration rate, the erythrocyte parameters and the correlations between them. The prevalence of anemia in patients with chronic kidney disease was of 51.35%. The hemoglobin concentration in patients with kidney transplantation (12.4 ± 2.7 g/dL) and in non-dialysis-dependent patients (11.7 ± 1.4 g/dL) is significantly different compared to the apparently healthy persons (14.6 ± 0.8 g/dL) (p<0.05). In the case of the non-dialysis-dependent patients who were not treated with erythropoiesis- stimulating agents we found positive associations between the glomerular filtration rate and the number of erythocytes (r = 0.71), hemoglobin (r = 0.65) and hematocrit (r = 0.73), as well as negative associations between creatinine and the number of erythrocytes (r = -0.72), hemoglobin (r = -0.86) and hematocrit (r = -0.88). In patients with kidney transplantation and anemia we observed positive correlations between erythropoietin and the number of erythrocytes (r = 0.69), between the glomerular filtration rate and the number of erythrocytes (r = 0.78) and erythropoietin (r = 0.97), as well as negative correlations between proteinuria and the number of erythrocytes (r= -0.89), hemoglobin (r= -0.72), hematocrit (r = -0.72), and erythropoietin (r = -0.67), and between creatinine and the number of erythrocytes (r = -0.75) and erythropoietin (r = -0.86).
Marian Pop, Raluca Pop and Anca Bacarea
Objective: To search for a dependency between blood Hounsfield Units values as measured by Computed Tomography and blood components with regards to hemoglobin oxygenated status, demographic data and acquisition parameters.
Methods: A retrospective study included a convenience sample of 144 SCJU patients who underwent routine thoracic CT examinations (Siemens Somatom AS 64 or 128 MDCT) in 2014 and had laboratory examinations done in nearby time. Variables included age, sex, mA, mV, aortic HU values, PA HU values, RBC, HTC, HGB, PLT, WBC blood sugar, PT and INR. Statistical analysis was performed using R version 3.1.1 and MedCalc 12.5 with a statistical significance factor of 0.05.
Results: The examined patients had an average age of 56.25, with a predominance of males (M:F=1.62). While there was a minor difference between HU values in Males vs Females (1.01 HU) it was proven as statistically significant (p=0.0051). No statistical significant difference between oxygenated/non-oxygenated blood (p=0.9636). Regression analysis found positive correlation between HU values and RGB, HGB and HCT with a significance level below 0.0001, the strongest being for the HGB level.
Conclusions: While HU values seem to carry multifactorial sources and the red blood cells being the most important an importance should be given to the fibrinoid plasma components which appear to bear negative impact.
Adina Stoian, Anca Bacârea, Anca Moţăţăianu, Mircea Stoian, Florina Gliga, Vladimir Bacârea, Carmen Duicu and Claudia Bănescu
The aim of this work was to study for the first time in Romania Insertion/Deletion (I/D) polymorphism of the Vascular Endothelial Growth Factor (VEGF) gene in a group of patients with established type 2 diabetes mellitus (DM) and diabetic peripheral neuropathy (DPN) compared with a control group.
This was a case-control study consisting of a group of 84 patients with type 2 DM and DPN, diagnosed by clinical neurological examination and electrophysiological nerve conduction studies and a control group of 90 healthy volunteers. For deoxyribonucleic acid (DNA) isolation, a DNA purification kit from Zymo Research was used. In vitro amplification of DNA sequences was achieved by polymerase chain reaction (PCR). Selective in vitro amplification of a DNA fragment of known sequence is based on the principle of extension of a primer (“primer and PCR amplicon”). DNA fragments were separated by gel electrophoresis. For proper viewing and interpreting of agarose gels Vilber Lourmat system was used. D allele frequency of VEGF was significantly higher in patients with diabetic peripheral neuropathy (53.57%) compared with controls (25%), p=0.0001.
There is a positive association between I/D polymorphism of VEGF gene and the presence of diabetic peripheral polyneuropathy. Our study suggests that D allele of VEGF gene is a risk factor for the occurrence of DPN.
Anca Bacârea, Claudia Bănescu, Ioan Macarie, Judit Beáta Köpeczi and Bogdana Dorcioman
Very few cases of chronic lymphocytic leukemia (CLL) presenting with extreme hyperleukocytosis are reported in the literature. We describe the case of a 66 years old woman, with newly diagnosed CLL presenting with extreme hyperleukocytosis of 774.2 x 109/liter, Rai stage III and Binet stage C. The patient has no comorbidities and the CIRS score (cumulative illness rating scale) is well below 6, with normal creatinine clearance. Some other interesting aspects related with this case are the atypical immunophenotype, the expression of Cyclin D1, and the B hepatitis viral infection, which made her diagnosis and treatment challenging. The patient was tested for NOTCH1 mutation and it was positive. There is important evidence that NOTCH1 mutations are associated with rapidly progressive disease and resistance to treatment. The distinction of CLL from mantle cell lymphoma (MCL) is not always easy because some MCLs may mimic CLL clinically, histologically, and/or phenotypically. The hepatitis B prophylaxis for viral reactivation was not available an in the end the patient was treated only with fludarabine and cyclophosphamide, without rituximab. CD200 should be introduced in the routine panel for flow cytometry to distinguish CLL from mantle cell lymphoma and NOTCH1 mutation is associated with poor prognosis and should be evaluated at diagnosis. CLL with extreme hyperleukocytosis presentation is very rare and sometimes an atypical CLL may represent a diagnostic pitfall.