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  • Author: Amar Zellagui x
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Open access

Saber Boutellaa, Amar Zellagui, Mehmet Öztürk, Chawki Bensouici, Özge Tokul Ölmez, Mouna Menakh and Mehmet Emin Duru

Abstract

In this study, phenolic compounds from Crithmum maritimum L. n-butanol soluble fraction were quantified and identified spectrophotometrically and by using HPLC-DAD technics. They equally investigated for their antioxidant potential utilizing six in vitro assays: DPPH, ABTS•+, O2 •−, Bleaching of β-carotene in linoleic acid, CUPRAC and Ferric reducing power. High amounts of total phenolics and flavonoids were recorded: 161.57± 0.479 μg GAeq.mg−1 and 31.56± 0.291 μg Qeq.mg−1 respectively. Nine compounds among them hydroxicinnamic acid and hydroxybenzoic acid derivatives, coumarins and flavonoids were identified. Chlorogenic acid known for their various pharmacological properties was detected as major compound of the extract. Rutin, vanillin, trans-2-hydroxycinnamic acid, ellagic acid, ferrulic acid, 6,7 dihydroxy coumarin, methyl 1,4 benzoquinone and trans-cinnamic acid were also detected. The extract was found to exhibit strong antioxidant capacities in all systems. Based on these results, it is right to conclude the n-butanol extract is promising source of natural antioxidants.

Open access

Abdelwahab Belkassam, Amar Zellagui, Noureddine Gherraf, Guido Flamini, Pier Luigi Cioni, Khellaf Rebbas and Tahar Smaili

Abstract

The essential oils obtained by hydrodistillation of aerial parts of Centaurea dimorpha Viv. were analyzed by means of gas chromatography-mass spectrometry (GC-MS). The main constituents were Caryophyllene oxide (09.88%), limonene (5.73%), tetradecanoic acid (5.68%), spathulenol (5.44%), methyl hexadecanoate (4.45%), and α-pinene (03.08%). To our best knowledge this is the first study of essential oils from the aerial parts of Centaurea dimorpha growing in Algeria. Moreover the essential oil and methanolic extract were screened for their possible in vitro antioxidant activity by DPPH free radical-scavenging test. The findings showed that the percentage inhibition is 10.67 and 77.01 respectively at a concentration of 1mole/L.

Open access

Ouroud Fellah, Samir Hameurlaine, Noureddine Gherraf, Amar Zellagui, Tahar Ali, Abdennabi Abidi, Muhammed Altun, Ibrahim Demirtas and Ayse SahinYaglioglu

Abstract

The aerial parts of T. gallica collected from three different locations (arid, humid and semi-arid) were extracted using ethyl acetate. The crude extracts were subjected to phenolic appraisal and antiproliferative activity using ELISA and xCELLigence assays. The total phenolic and flavonoids were evaluated using appropriate techniques to give a yield of total phenolics ranging between 238.46 and 348.56 mg GAE (Gallic acid equivalent)/g dry weight extract. The flavonoids yield was found to vary from 36.6 to 103.14 mg QE (quercetin equivalent)/g dry weight extract. Moreover, the extracts were tested against rat brain tumor (C6) and human cervix carcinoma (HeLa) cell lines and displayed important differences in activity. These disparities highlighted the effect of climatic factors as quality determinants of secondary metabolites and therefore as a key control of the biological therapeutic effect.

Open access

Djamila Maanani, Narimane Segueni, Salah Rhouati, Yavuz Selim Çakmak, Maltem Asan-Ozusaglam, Abdghani May, Amar Zellagui and Salah Akkal

Abstract

This study was carried out to determine the phenolic contents as well as to evaluate the antioxidant and antimicrobial activities of Genista microcephala. Total phenolic and flavonoid compounds were quantified. Antioxidant activity was evaluated by various in vitro tests, including Ferric Ion Reducing Activity (FRP), Cuprac Ion Reducing Antioxidant Capacity (CUPRAC), and total antioxidant capacities (TAC). The antimicrobial activity of ethyl acetate and butanolic extracts of G. microcephala in addition of fatty acids content of different parts of the studied plant was evaluated against human pathogenic bacteria, fish bacteria and two yeast. The used methods were agar diffusion method and the determination of minimal bactericidal and fungicidal concentrations (MBC and MFC). No significant difference was found between the two used solvents on total phenolic content (TPC). While, difference in total flavonoid content (TFC) were found to be significant. Ethylene acetate extract was the most actif regarding antioxidant assay. The tested activity seems to be due to the high content of this extract on isoflavonoids. The tested extracts demonstrated the lowest inhibition concentration for B.cereus RSKK 863.