Alina Elena Cuvliuc, Gheorghe Emil Mărginean, Alexandru Kelemen and Livia Vidu
The aim of this scientific paper is to reveal the trends of the parameters that quantitatively and qualitatively assess the milk production of the Holstein livestock exploited in Romania. The data used here came from 59 dairy farms throughout Romania and accounted for over 34,000 complete lactations from the period between 2012 and 2017. The comparison of currently processed data has been done with the results obtained over the last 20 years by another Romanian author. The following parameters, expressed per total lactation and normal lactation (305 days), were used to assess milk production: the length of lactation, quantitative milk production, quantitative fat production, and the concentration of milk in crude fat. The statistical processing of data was done using the Excel software. In general, in the last 20 years, there has been asignificant improvement in milk production of the Holstein livestock from Romania dairy farms.
Alexandru Robert Vlad, Gabriel Hancu, Hajnal Kelemen, Diana Ciurcă and Amelia Tero-Vescan
Sibutramine is a chiral anti-obesity drug which decreases food intake and increases energy expenditure. In therapy it is used as a racemic mixture; however both pharmacokinetic and pharmacodynamic data have revealed enantioselective behavior of sibutramine and its major active metabolites. Several chromatographic and electrophoretic analytical methods have been published so far for the chiral determination of sibutramine from pharmaceutical preparations and biological samples. The current paper aims to provide a systematic review of the stereochemical aspects and analytical methods used for the enantiodetermination of sibutramine and its active enantiomers covering the last 15 years.
Adriana Modroiu, Gabriel Hancu, Robert Alexandru Vlad, Ștefana Stăcescu, Ruxandra Soare and Hajnal Kelemen
A rapid, simple and sensitive capillary zone electrophoresis method was developed for the simultaneous determination of amlodipine besylate and telmisartan, in fixed-dose combinations, through the utilization of a UV photodiode array detector. Electrophoretic parameters such as buffer concentration and pH, system temperature, applied voltage and injection parameters, were optimized in order to improve the efficiency of the separation. The best results were obtained when employing fused silica capillary (48 cm length X 50 μm ID) and 50 mM phosphate buffer electrolyte at pH 4.50, + 25 kV applied voltage, as well as 25°C system temperature. The separation was achieved in approximately 3 minutes, with a resolution of 4.90, while the order of migration was amlodipine followed by telmisartan. The analytical performance of the method was verified with regard to linearity, precision and robustness. In addition, the limit of detection and the quantification were calculated.