This research aims to evaluate the properties of cotton single jersey knitted fabrics (SJKF) produced from cotton/spandex yarns at different Lycra states. So, four different SJKF were produced, namely 100% cotton, cotton with additional Lycra (full-platted), core, and dual-core-spun (DCS) yarns with the same loop length. The thermal comfort properties, fabric recovery, total hand value (THV), moisture management parameters, and air permeability were measured. The experimental results showed that the use of DCS yarns in the SJKF improves the fabric elastic recovery by 100%. The obtained values of air permeability, THV, and overall moisture management capacity of stretched SJKF are lower than 100% cotton fabric sample. Thermal absorptivity of core and dual-core samples increased by 27% and the water vapor permeability decreased by 18% compared to 100% cotton fabric sample.