Patrycja Sujka-Kordowska, Agnieszka Malińska and Maciej Zabel
It is well established that angiogenesis is necessary in solid tumours development. Interesingly, the role of angiogensis in haematological malignancies has been also recognized. Recent publicationts indicate that microvessel density in bone marrow and angiogenic factors like endoglin, placental growth factor and cyclooxygenase 1 are increased in lymphoproliferative disorders and suggest that angiogensis is a part of pathomechanism of these malignancies. However, it has not been identified how angiogenesis can be involved in progression of haematological disorders. There is a strong need for further investigations in this area, since antiangiogenic therapy is a potential adjunct to conventional therapy of lymphoproliferative disorders.
Aneta Konwerska, Beata Janik, Agnieszka Malinska, Wojciech Witkiewicz and Maciej Zabel
Recently, the most significant studies in the field of angiogenesis are related to trials concerning the evaluation of the prognostic significance of endothelial markers. By using an estimation of the expression of endothelial cell markers, immunohistochemical studies can assess for the continuity of the vascular intima, as well as allow for the distinction of early, late and mature forms of endothelial cells. The determination of the endothelial cell phenotype using markers such as CD34, CD31, CD133, e-NOS and von Willebrand factor may provide a valuable tool for the monitoring of both normal physiology as well as neoplastic diseases.
Krystyna Filipiak, Agnieszka Malińska, Dariusz Krupa and Maciej Zabel
The dynamic development of technical sciences and informatics makes now possible acquisition of microscopic images of histological sections, not only using digital cameras, but also through specialized devices called scanners. The digitalized images stored in a computer storage device are called virtual slides and, together with special software, are known as virtual microscopy. The virtual slides can be analyzed on a computer screen by panoramic viewing or using a detailed image examination at higher magnification. In many research and education institutions in both the U.S. and Europe, the virtual microscopy is used for teaching and training purposes. In the academic year of 2009/10, Department of Histology and Embryology, University of Medical Sciences in Poznan, as one of the first in Poland, has created a virtual database for educational purposes. This database created by archiving the traditional images of histological slides in the form of digital images. So far, more than 130 virtual slides have been acquired and catalogued in 24 thematic folders, available for medical students participating in histology, embryology and cell biology courses.
Telepathology is the second branch which uses virtual microscopy. Virtual microscope allows to discuss and resolve medical/diagnostic problems with the use of telecommunication systems and information technology. The existing internet platforms offer access to virtual microscopes and virtual slides. In June, 2011 the Center of Morphologic Images Archivization and Digital Database of Microscopic Pictures in the Department of Histology and Embryology, Poznan University of Medical Sciences has launched an online platform (www.caom.pl), aimed to provide the central database of scanned histological sections of physiological tissues, and pathological, rare and sporadic lesions, including tumor