Search Results

1 - 4 of 4 items

  • Author: Agnieszka Kubiak x
Clear All Modify Search

Abstract

The paper presents hydrographic changes in a river system and their influence on the legal classification of watercourses in Poland. As a case study, the watercourse Motwica, right tributary of the river San has been analysed. The main objective of this paper is an attempt to analyse whether the Motwica should be classified as flowing or standing water and the legal grounds for such classification in the Water Law Act. On the base of archival and contemporary cartographic materials’ analysis it has been determined that the Motwica should not be classified as natural watercourse because its significant part flows in an artificial channel.

Oxygen conditions and trophic state of Lake Głębokie (Szczecin, Poland) in the years 2008-2010

The research in Lake Głębokie was conducted in the years 2008-2010. Sampling from three selected sites was designed in such a way that vertical profiles of the characteristics of interest were obtained. Measurements were performed to determine the content of dissolved oxygen, oxygen saturation expressed as a percentage, water temperature, total phosphorus, chlorophyll a, dry seston mass, and Secchi disk depth. During the course of a year, water temperature varied from 0.58°C to 24.72°C. However, a permanent thermal stratification was not observed in the summer season due to complete water mixing. Dissolved oxygen concentrations in the water varied within a range that started at hard-to-measure insignificant values and reached up to 9.63 mg O2 l-1, while oxygen saturation reached a maximum of 116% O2. At the same time, it was observed that oxygen conditions gradually worsened as the depth became greater. The largest oxygen deficits occurred in the winter season of 2009-2010. The oxygen conditions were typical for an eutrophic lake. Its high trophic state was confirmed by an analysis performed using the method proposed by Carlson (1977) as well as by an analysis performed according to the directives of the OECD (1982). The trophic state of this lake is borderline eutrophic and hypertrophic. The oxygen conditions found in this lake ought to be seen as not beneficial to fish.

Streszczenie

W procesie przygotowania komórek krwiotwórczych (k.k.) do transplantacji istotne jest wdrożenie oraz utrzymanie procedur pozwalających na zachowanie mikrobiologicznego bezpieczeństwa przeszczepu. Do głównych źródeł zanieczyszczenia przeszczepu należy: powietrze, personel, woda oraz materiał. Kontaminacja przeszczepu może mieć miejsce na etapie pozyskania, przetwarzania oraz dystrybucji k.k. Jakość materiału transplantacyjnego powinna być kontrolowana na wszystkich kluczowych etapach jego przetwarzania. Bankowanie k.k. może odbywać się w systemie otwartym lub zamkniętym. System przetwarzania k.k., otwarty lub zamknięty, znacząco wpływa m. in. na kryteria kwalifikacji pomieszczeń oraz przygotowania pracowników do pracy. Jednakże niezależnie od systemu przetwarzania k.k., kontrola mikrobiologiczna powinna obejmować zarówno materiał transplantacyjny, jak i środowisko jego przewarzania. Formalne wymogi dla banków komórek zawarte są w Rozporządzeniach Unii Europejskiej, Ustawie Transplantacyjnej oraz Rozporządzeniach Ministra Zdrowia. Wdrożenie oraz utrzymanie procedur odpowiedniego przygotowania pracowników, sprzętów oraz pomieszczeń do pracy w znaczący sposób wpływają na zmniejszenie ryzyka kontaminacji przeszczepu, a tym samym na kliniczne parametry leczenia transplantacyjnego.

Abstract

The objective of this paper is to present the process of the national and international accreditation leading to the establishment of the first certified chimeric antigen receptor T (CAR-T) Cell Unit in Poland on the basis of the Department of Hematology and Bone Marrow Transplantation in Poznan University of Medical Sciences and first successful CAR-T therapy in Poland. During 12 months from the initial decision to establish the CAR-T Cell Unit to the application of CAR-T cell treatment in the first patient, the center had to undergo the multidisciplinary external and internal training, as well as the adaptation of multiple procedures within the Transplant Unit and Stem Cell Bank. In order to get accreditation for the implementation of CAR-T cell therapy, an initial training of the team involved in handling cellular products and patient care was organized and updated as a continuous process. The Department fulfilled the site-selection international criteria. The first patient diagnosed for refractory/relapsed DLBCL was qualified, and finally CAR-T cells were administered with successful clinical outcome.