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Lidia Radko, Maria Minta, Agnieszka Jasik, Sylwia Stypuła-Trębas and Jan Żmudzki

Abstract

The present study assessed the sensitivity of immature hamster uterotrophic assay to reference oestrogen agonists/antagonists in order to develop a sensitive model for evaluation of endocrine-active compounds in diets. After performing a baseline for control animals, the sensitivity of immature females (postnatal day 18) to reference compounds was evaluated in a three-day uterotrophic assay. The absolute and adjusted dry uterine weights, fold induction over control for absolute wet uterine weight, and wet uterine weight/body weight ratio (%) were used as endpoints. The significantly active doses for reference oestrogens were as follows: 0.6 μg/kg for 17α-ethinyloestradiol (s.c.); 1 μg/kg/day (s.c.) and 40 μg/kg (p.o.) for diethylstilboestrol; 40 mg/kg (s.c.) and 160 mg/kg (p.o.) for bisphenol A. Co-treatment with tamoxifen at a dose of 1 mg/kg significantly antagonised the uterotrophic effect induced by 1 μg/kg 17α-ethinyloestradiol, and showed the attenuated proliferative effect in histopathological examination. We found immature hamster uterotrophic assay as a sensitive model that could be a good alternative to the rat assay.

Open access

Jacek Karamon, Magdalena Larska, Agnieszka Jasik and Bartosz Sell

Abstract

A 3-year-old female fallow deer was subjected to the necropsy and virological testing, due to a suspected infectious disease in the herd of farmed deer in the Southeastern region of Poland. The animal was found negative for the presence of BVDV, BoHV-1, BTV, and EHDV antibodies and BVDV antigen. The toxicological examination did not reveal any coccidiostats, mycotoxins, rodenticides, carbamate pesticides, and organophosphorus pesticides. The flukes found during postmortem examination were first characterised microscopically as Fascioloides magna and later their identity was confirmed by PCR and sequencing. The autopsy revealed lesions characteristic for F. magna infection, including different size cystic spaces in the liver, filled with brownish mucous fluid and flukes, and black pigment covering the surface of parietal and visceral peritoneum with the highest concentrations localised next to the liver. The changes observed in the liver tissue were typical of liver cirrhosis. The results demonstrated that in Poland, where the cervid farming is developing dynamically, the problem of fascioloidosis is present and may probably exert a significantly negative influence on the productivity of such farms if no antiparasitic treatment is performed.

Open access

Anna Śmiech, Wojciech Łopuszyński, Brygida Ślaska, Kamila Bulak and Agnieszka Jasik

Abstract

Introduction: Breed predisposition to cutaneous mast cell tumours (MCT) in a population of dogs in Poland affected by various skin tumours was assessed, and the distribution of MCT characteristics such as histological grading, sex, age, and location, in predisposed breeds was evaluated.

Material and Methods: The retrospective epidemiological study included 550 dogs affected by cutaneous MCTs with a reference group of 2,557 dogs diagnosed with other skin tumours.

Results: A univariable logistic regression analysis was performed to determine the odds ratios (ORs) with 95% confidence intervals. The risk of high-grade MCTs was the highest for Shar-Peis (OR: 26.394) and American Staffordshire Terriers (OR: 2.897). Boxers (OR: 6.619), Labrador Retrievers (OR: 2.630), French Bulldogs (OR: 2.050), Golden Retrievers (OR: 1.949), and American Staffordshire Terriers (OR: 2.592) were mainly affected by low-grade MCTs. The high risk of MCT was calculated to be at the age of 4–6 years for Labrador Retrievers (OR: 2.686) and 7–10 years for Boxers (OR: 2.956) and French Bulldogs (OR: 9.429). MCTs were significantly more often located on the trunk in French Bulldogs (OR: 4.680), American Staffordshire Terriers (OR: 2.520), and Labrador Retrievers (OR: 1.948). There was no statistically significant correlation between gender and the occurrence of MCTs in the breeds.

Conclusions: The breed-predicated differences in the clinical course of MCTs suggest a genetic background for the tumours.

Open access

Anna Kycko, Agnieszka Jasik, Łukasz Bocian, Iwona Otrocka-Domagała, Mateusz Mikiewicz, Anna Śmiech, Wojciech Łopuszyński, Izabella Dolka, Marcin Nowak and Janusz A. Madej

Abstract

Introduction: Apocrine sweat gland carcinomas (ASGCs) are malignant neoplasms of dogs and other animals, rarely reported worldwide. The aim of this study was to summarise the occurrence of this cancer in a population of dogs in Poland between 2009 and 2014 with regards to histological features and body location of the tumours, as well as age, sex and breed of the cancer-affected dogs.

Material and Methods: The study involved 40 canine ASGC cases diagnosed in five national veterinary pathology laboratories. The material was processed according to routine histological methods.

Results: Histological types of the tumours involved simple and complex apocrine carcinoma of cystic/papillary (62.5%), solid (15%), and tubular type (12.5%), as well as apocrine ductal carcinoma (10%). The epidemiological analysis revealed peak incidence of the cancer in dogs between 8 and 14 years of age, with the most commonly affected sites being forelimbs and thorax. The highest number of the cancer cases was diagnosed in mixed breed dogs and German Shepherds; no sex predilection was noted.

Conclusion: To the authors’ knowledge, this is the first report recounting the study on canine malignant apocrine sweat gland tumours in Poland providing detailed phenotypical and histological data, which are otherwise rarely described in veterinary literature. This type of cancer appears to be diagnosed more frequently in dogs than in humans. Being an easily accessible material for research, canine ASGCs might serve as a relevant animal model for studies related to pathogenesis of sweat gland tumours.

Open access

Sławomir Jaworski, Ewa Sawosz, Marta Grodzik, Marta Kutwin, Mateusz Wierzbicki, Karolina Włodyga, Agnieszka Jasik, Michał Reichert and Andrè Chwalibog

Abstract

The objective of this study was the morphological and structural characterisation of glioblastoma multiforme grown in ovo. Glioma cells U87 and U118 were implanted in the chorioallantoic membrane (CAM) of chicken egg. After 10 days of incubation, tumours were resected for further analyses. Culturing two types of glioblastoma tumours from U87 and U118 cell lines has shown a number of differences in their morphology, histology, and ultrastructure. CAM assay proved to be a useful tool for studying glioblastoma growth. The model provides an excellent alternative to current rodent models and could serve as a preclinical screening assay for anticancer molecules. It might increase the speed and efficacy of the development of new drugs for the treatment of glioblastoma.

Open access

Michał Reichert, Wojciech Kozaczyński, Teresa Agnieszka Karpińska, Łukasz Bocian, Agnieszka Jasik, Anna Kycko, Małgorzata Świątkiewicz, Sylwester Świątkiewicz, Iwona Furgał-Dierżuk, Anna Arczewska-Włosek, Juliusz Strzetelski and Krzysztof Kwiatek

Abstract

Histopathological examination of liver, kidney, spleen, pancreas, duodenum, jejunum, skeletal muscle, and bursa of Fabricius samples, collected from broiler chickens, laying hens, fattening pigs, and calves fed genetically modified corn MON 810 and soybean meal MON-40-3-2 (Roundup Ready, RR), was performed The examination showed no significant differences between the control animals fed diets containing no genetically modified feeds and animals fed genetically modified feeds. In some cases, congestion of parenchyma and focal lymphoid cell infiltrations were observed in all dietary groups, including controls, and therefore, it was assumed that the lesions were not associated with the feeding transgenic feeds.