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Open access

Aneta Grabowska, Edward Kunicki, Agnieszka Sękara, Andrzej Kalisz and Renata Wojciechowska

Summary

Modifications in growing techniques can affect the yield and nutritional quality of various cultivated plants. Among them, the use of biostimulants is environmental friendly method of stimulating crop productivity, stress resistance, and affecting yield or chemical composition of the plants. The aim of the investigation was determining of the effect of biostimulant treatment on yield and its quality of carrot grown for summer harvest. The experiment was carried out in 2009-2011 in the experimental station of the University of Agriculture in Krakow, south Poland. Two experimental factors were taken into consideration: (1) cultivar: Nandrin F1 and Napoli F1 (2) dose of Aminoplant (foliar application): 1.5 and 3.0 dm3∙ha-1 and control (without Aminoplant). Total and marketable yield, root length, its diameter, leaf mass and leaf : root mass ratio were assessed. The dry matter, soluble sugar, carotenoids and nitrate ions contents were analyzed as main determinants of carrot nutritional quality.

Aminoplant influenced not only carrot productivity, but mainly chemical composition of the roots. The present results also suggest that carrot reaction to biostimulant treatment was depended on a cultivar more than on environmental conditions in particular growing seasons. The significant effect of Aminoplant in a dose of 1.5 dm3∙ha-1 on the yield of roots and leaf rosette mass of ‘Nandrin F1’ appeared only in the first year of the experiment. Spraying with Aminoplant in a dose of 3.0 dm3∙ha-1 significantly increased the soluble sugars content in carrot roots of both cultivars but only in 2011. Dry matter content was also affected by biostimulant treatment mainly for ‘Napoli F1’, which showed the lowest dry matter content when sprayed with Aminoplant in a dose of 1.5 dm3∙ha-1. In 2010 control plants contained the greater amount of carotenoids, while in next year roots of plants treated with Aminoplant in a dose of 3.0 dm3∙ha-1 had more these compounds. The significant effect of Aminoplant on nitrates content in carrot roots was observed but were not repeatable in the experimental years, so different climatic conditions modified carrot reaction on biostimulant spraying.

Open access

Edward Kunicki, Aneta Grabowska, Agnieszka Sękara and Renata Wojciechowska

Abstract

The aim of the present study was to investigate the influence of spraying with Aminoplant on the yield of two spinach cultivars in the spring and autumn cultivations. The experiment was carried out in 2008 and 2009 in the experimental station of the University of Agriculture in Krakow, Poland. Three factors were taken into consideration: (1) cultivar: ‘Rembrandt F1’ and ‘Spiros F1’; (2) time of cultivation: spring and autumn; (3) dose of Aminoplant: control (without Aminoplant), 1.5 dm3 ha-1 and 3.0 dm3 ha-1. The spinach yield was dependent on the time of production and cultivar type, and ranged between 18.6-44.8 t ha-1. Both cultivars yielded better in autumn cultivation. Spraying with Aminoplant had no effect on spinach yield. Dry matter content in spinach leaves was between 6.3-11.2 g 100 g-1. Spinach grown in the autumn had a greater content of dry matter in comparison to the spring cultivation. In 2009, ‘Rembrant F1’ was characterized by greater dry matter content than ‘Spiros F1’. Aminoplant in a dose of 3.0 dm3 ha-1 lowered dry matter content in spinach leaves as compared to the control. The nitrate content in spinach was differentiated (558-3506 mg NO3 kg-1 f.m.) and depended on the time of cultivation, the cultivar, and the Aminoplant dose.

Open access

Marzena Rola-Łuszczak, Agnieszka Grabowska, Bogusław Szewczyk and Jacek Kuźmak

Abstract

Introduction: Field isolates of bovine leukaemia virus (BLV) show the presence of a few amino acid substitutions in major conformational G and H epitopes on surface glycoprotein gp51. Potentially, these substitutions can affect the 3D structure of these epitopes leading to their diminished immunoreactivity. The aim of this study was to express three gp51 glycoproteins carrying mutated epitopes as recombinant baculovirus proteins in insect cells to test their immunoreactivity with bovine sera.

Material and Methods: Env gene chimeras encoding mutated epitopes G and H in the env backbone of BLV FLK strain were constructed, cloned into pFastBac1 vector, and expressed in baculovirus.

Results: The presence of recombinant gp51 protein in Sf9 insect cells was confirmed using monoclonal antibodies. ELISA tests were developed to check the immunoreactivity of recombinant protein with bovine sera.

Conclusion: Recombinant gp51 proteins with altered G and H epitopes can be used for further studies to analyse the serological response of bovine sera towards BLV antigenic variants.

Open access

Beata Starzyńska, Agnieszka Kujawińska, Marta Grabowska, Jacek Diakun, Ewa Więcek-Janka, Lars Schnieder, Nadine Schlueter and Jan-Peter Nicklas

Abstract

In this article the authors present the methodology adopted and the results obtained in the first stage of the research encompassing focus group interviews (FGI) about the needs of public transport users in a selected city (Poznań). The elicitation and assessment of the requirements were carried out for three groups of people with disabilities using public transport in the city of Poznań: blind and partially sighted people, deaf and hearing-impaired people, as well as people with locomotor dysfunctions. A study carried out on the basis of a scenario especially designed for the FGI purpose has made it possible to identify barriers for people with disabilities and, consequently, to formulate their pre-trip,on-trip and post-trip requirements when it comes to urban public transport services. The results will be used to construct a questionnaire to be used further on in the project.

Open access

Wiesława Młodawska, Patrycja Mrowiec, Beata Grabowska, Joanna Waliszewska, Joanna Kochan, Agnieszka Nowak, Anna Migdał, Wojciech Niżański, Sylwia Prochowska, Agnieszka Partyka, Marcin Pałys, Teresa Grega and Józef Skotnicki

Abstract

Dermal fibroblasts are commonly used as donors of genetic material for somatic cell nuclear transfer in mammals. Basic fibroblast growth factor (bFGF) is a cytokine that regulates proliferation and differentiation of different cell types. The study was aimed at optimizing the cell culture protocol for cat dermal fibroblasts by assessing the influence of culture media and different doses of bFGF on proliferation of fibroblasts and their viability in terms of cell banking and somatic cloning of felids. In Experiment I, skin biopsies of domestic cats were cultured in DMEM (D) and/or DMEM/F12 (F), both supplemented with 5 ng bFGF/ml (D-5, F-5, respectively). After the primary culture reached ~80% of confluency, the cells were passaged (3–4 times) and cultured in media with (D-5, F-5) or without (D-0, F-0) bFGF. To determine the optimal doses of bFGF, in Experiment II, secondary fibroblasts were cultured in DMEM with 0 (D-0), 2.5 (D-2.5), 5 (D-5) or 10 (D-10) ng bFGF/ml. The results showed that in D-5 the cells proliferated faster than in D-0, F-5 and F-0. Due to their poor proliferation, passages IV were not performed for cells cultured in F-0. In experiment II, a dose-dependent effect of bFGF on proliferation of cat dermal fibroblasts was found. In D-5 and D-10, the cells exhibited higher (P<0.05) proliferation compared with D-0. In D-2.5 the cells showed a tendency to proliferate slower than in D-5 and D-10 and at the same faster than in D-0. In conclusion. DMEM supplemented with bFGF provides better proliferation of domestic cat dermal fibroblasts culture than DMEM/F12. Supplementation of culture medium with bFGF has a beneficial effect on cat dermal fibroblast proliferation and could be recommended for addition to culture media.

Open access

Barbara Felic, Magdalena Grabowska, Agnieszka Krzętowska, Marlena Piekut and Renata Walczak

Abstract

The article presents a problem and differences of financial liquidity and profitability. The survey of financial liquidity and profitability, underwritten by the College of Economics and Social Sciences of Warsaw University of Technology, was conducted on Płock Subregion enterprises. The survey concerned cash flow management, receivables controlling, trade credits, different payments and receivables. The results of the conducted survey indicate that the majority of companies from the subregion of Płock suffer several problems and despite a wide range of possibilities of using different tools for the management of receivables, the respondents do not use them efficiently. The survey has shown that most of them applies only the most popular, simplest and cheapest solutions, but, consequently the least efficient.

Open access

Marlena Piekut, Renata Walczak, Barbara Felic, Agnieszka Krzętowska, Magdalena Grabowska, Magdalena Kludacz and Grzegorz Chybicki

Abstract

The aim of the paper is the analysis of the household use of durables in Poland and other European countries. Relatively, the highest amount of money for household furnishing with durables was spent by the Bulgarians and the Italians (more than 7% of the total household expenses). In Poland, the highest expenditure occurred for the wealthiest households and those where the head of the household had a university degree while the least money was spent in numerous-family households. The best equipped households (quantity wise) were such that had one or two children and those where the household head had a university degree. The cluster analysis allowed distinguishing household groups of similar durables ownership level. These groups may be treated as consumer segments. The presented analyses also indicate the trends of the Polish consumers regarding the possession of durables.