Beata Łabaz, Adam Bogacz and Bartłomiej Glina
Beata Łabaz, Adam Bogacz and Cezary Kabała
Large-scale river regulation, drainage and intense farming in the Barycz valley initiated in 17th century activated a transformation of the initial alluvial and swamp-alluvial soils. Soils on the Holocene flooded terraces have deep, acid humus horizons (umbric) and gleyic properties at shallow depth, but have no stratification of parent material to a depth of 100 cm. Despite the location in the floodplain, soils cannot be classified as black-earth alluvial soils (mady czarnoziemne) using the criteria of Polish soil classification (2011). The soils on the Pleistocene non-flooded terraces have a deep, base-saturated humus horizon (mollic) and gleyic properties in the lower part of soil profile, which allows to classify them as the black earths (czarne ziemie). Prominent stratification of the parent material well preserved in these soils has no influence on their classification (due to the age sediments). Almost all humus horizons of these soils meet the definition of anthric characteristics, and more than half of the studied soils can be classified as culturozemic soils - rigosols - which emphasises the important role of man in the transformation and gaining of morphological features of these soils. The lack of precise criteria for identifying soil types in the chernozemic order of the Polish soil classification (2011) causes difficulties in the classification of soils on the river terraces, in particular, in distinguishing between black-earth alluvial soils and black earths.
Maria Andrzejczak, Adam Bogacz, Klara Tomaszewska and Magda Podlaska
The aim of the study was to show the impact of the peat extraction on the development and properties of organic soils and plant habitat in post-extraction sites. The study was conducted in the complex of the Trzcińskie Mokradła Peatlands (Sudetes Mts., SW Poland). The Trzcińskie Mokradła Peatlands began to form in Preboreal (10960–9330 ±50BP) so that they are one of the oldest peatlands in the Sudetes. We analyzed 8 soil profiles (42 samples). Peat forming process there is still active in the moderate or strong degree (PtII-PtIII). The floristic composition of the studied areas was typical of transition peatlands. Successive dry and moist periods were observed in the developed of organic soils. The time gaps in peat profiles covering hundreds of years prove their extraction in the past.
Bartłomiej Glina, Adam Bogacz and Przemysław Woźniczka
The aim of this work was to determine the intensity of nitrogen mineralization in forestry drained ombrotrophic peatland soils in the Stołowe Mountains National Park, SW Poland. Additionally discussion about the shallow organic soils classification according to Polish Soil Classification (2011) is presented. For the study three research transects were established on forestry drained ombrotrophic peatlands in the Stołowe Mountains. Each of the transect consisted of four (site A and B) or five (site C) sampling plots. Sampling was conducted in the year 2012. The soil samples for the basic soil properties analysis were sampled in April, whereas undisturbed soil samples were collected in stainless steel rings (100 cm3) every 10 cm in April (spring), July (summer) and October (autumn) to show the seasonal dynamics of nitrogen mineralization. Statistical analysis showed that the content of N-NH4 was mainly determined by actual soil moisture and precipitation rate, whereas the content of N-NO3 was positively correlated with air temperature. Among investigated peatlands the highest concentrations of mineral nitrogen forms was observed in the Długie Mokradło bog, situated on the Skalniak Plateau-summit. Additionally, the results obtained showed that implementation of new subtype: shallow fibric peat soils (in Polish: gleby torfowe fibrowe płytkie) within the type of peat soils (in polish: gleby torfowe) should be considered during developing of the next update of Polish Soil Classification.
Adam Bogacz, Dominika Dzięcioł, Bartłomiej Glina and Leszek Gersztyn
Gleby Organiczne na Renaturyzowanym Torfowisku "Niknąca Łąka" w Parku Narodowym Gór Stołowych
Bartłomiej Glina and Adam Bogacz
Despite a large number of organic soil types and subtypes in the Polish Soil Classification the problems of organic soils classification are still very common. In relation to mountain organic soils, in particular. The aim of this paper is to discuss the most common problems related to mountain organic soils classification according to the Polish Soil Classification. Based on authors’ own research and literature studies mentioned problem was described. This work allows to define some new proposals, which should be considered during developing of the next update of the Polish Soil Classification (PSC). The most important proposals related to: criteria for organic materials and organic soils, taxonomy position and criteria for shallow organic soils and new definition of mineral material admixture in organic soils.
Cezary Kabała, Jarosław Waroszewski, Adam Bogacz and Beata Łabaz
O Specyfice Bielic Górskich
Katarzyna Szopka, Cezary Kabała, Anna Karczewska, Paweł Jezierski, Adam Bogacz and Jarosław Waroszewski
Differentiation of soil organic carbon (SOC) concentrations and pools in topsoil horizons of forest soils in the Karkonosze Mountains was examined in relation to environmental and human-induced factors, with special focus on altitudinal gradient, related climatic conditions, and a zonality of vegetation. The samples were collected from the forest litter and soil layers 0–10 cm and 10–20 cm, in 621 plots arranged in a regular network of monitoring established in the Karkonosze National Park. The concentrations of SOC were determined in laboratory and used for calculation of SOC pools. Four elevation zones were distinguished for analysis: 500–750 m, 750–1000 m, 1000–1250 m, and >1250 m. The concentrations of SOC in forest litter (38.3–44.1%) showed an insignificant increasing trend with altitude. The concentrations of SOC in the layers 0–10 cm and 10–20 cm, were in a very broad range 0.27–47.6%, thus indicating a high differentiation, and also tended to insignificantly increase along with altitude. The largest share of accumulated SOC pools was proved to be present in the layer 0–10 cm, except for the highest zone >1250 m in which forest litter contains slightly larger amounts of SOC. The pools of SOC accumulated in the 20 cm thick topsoil and forest litter turned out to vary considerably (3.6–58.2 kg·m−2), but the mean values and medians in particular elevation zones fall in a narrow range 10.5–11.9 kg·m−2, close to the values reported from the Alps. The lack of statistical significance of reported tendencies was explained by a monitoring sites-oriented random soil sampling, i.e. in forest stands of various age, species-composition and degradation degree.
Anna Bogacz, Marlena Wolek, Bogna Juskowiak, Monika Karasiewicz, Adam Kamiński, Izabela Uzar, Anna Polaszewska, Zofia Kostrzewa and Bogusław Czerny
Introduction: Breast cancer is the most common malignant cancer among women. Both drug resistance and metastasis are major problems in the treatment of breast cancer. Therefore, adjuvant therapy may improve patients’ survival and affect their quality of life. It is suggested that epigallocatechin gallate (EGCG) which is well known for its chemopreventive activity and acts on numerous molecular targets may inhibit the growth and metastasis of some cancers. Hence, discovering the metastatic molecular mechanisms for breast cancer may be useful for therapy.
Objective: The aim of the study was to determine the effect of EGGC on the mRNA expression level of genes such as ZEB1, ABCB1, MDM2, TWIST1 and PTEN in MCF-7 breast cancer cells.
Methods: MCF7/DOX were cultured in the presence of 0.2 μM DOX and EGCG (20-50 μM). The mRNA expression level was determined by real-time quantitative PCR using RealTime ready Custom Panel 96 kit.
Results: Our results showed an important increase (about 2-fold for 20 μM EGCG + 0.2 μM DOX and 2.5-fold for 50 μM EGCG + 0.2 μM DOX, p<0.05) in ZEB1 expression levels. In case of ABCB1 gene lack of influence on the mRNA level was observed (p>0.05). We also observed significant decrease of ZEB1 expression in MCF7 cells with 20 μM and 50 μM EGCG (p<0.05). In addition, EGCG (20 μM) caused an increase of MDM2 and PTEN mRNA levels in almost 100% (p<0.05) and 40% (p>0.05), respectively. Lack of the influence of EGCG was noted for the TWIST1 gene expression. In case of MCF7/DOX we showed an increase of mRNA level of PTEN gene about 50% (p<0.05).
Conclusions: These results suggest that EGCG may be potentially used in adjuvant therapy in the breast cancer treatment.