Profitable prospective of rocket plant is progressively growing recently. Hence an experiment was conducted in open field to evaluate the effect of chicken manure, nano iron (Fe), nano zinc (Zn) and combination of them on morphological, fresh weight and seed yield, photosynthetic rate, transpiration rate, water use efficiency and chemicals constituents represented in macro and micro elements, plant pigments, total phenolics, total carbohydrate, alkaloids, tannins, flavonoids, ascorbic acid, crude protein, total fatty acids, indole acetic acid (IAA) and abscisic acid (ABA) hormones, oil seed yield and methylthiobutyl- isothiocyanate as main sulphur content in essential oil of Eruca sativa Mill. compared to chemical fertilisers (NPK) as control. Results revealed that, nano Fe and Zn treatments either alone or in combination with manure had the upper hand, where significantly increased almost all parameters under study in comparison with control. The outcomes of present research gave emphasis to global warning about pollution of chemical fertilisers and safety production.