Properties of materials as determined by inverse gas chromatography
The application of inverse gas chromatography (IGC) in the determination of the physicochemical properties of various materials is presented and discussed. The special attention is focused on the determination of solubility parameters.
Surface modification of bioceramics by PEG grafting
Surface modification of Hydroxyapatite (HAp) and β-Tricalcium phosphate (β-TCP) powders was performed using hexamethylene diisocyanate (HMDI) as a coupling agent. Polyethylene glycol (Mw = 2000) was grafted to the surface of these ceramic materials. Different methods were used to characterize modified surfaces. Fourier transform infrared (FT-IR) and Attenuated Total Reflection Fourier Transform Infrared (FTIR/ATR) technique analyses confirmed the modification reaction on HAp and β-TCP surfaces. BET isotherm analysis showed the changes in textural properties of materials after modification. Elemental analysis was performed to confirm a presence of selected elements from modifier and coupling agent. Examination of dispersion stability of materials in different solvents show better stability for samples of β-TCP than HAp.
E Roemer, H Schramke, H Weiler, A Buettner, S Kausche, S Weber, A Berges, M Stueber, M Muench, E Trelles-Sticken, J Pype, K Kohlgrueber, H Voelkel and S Wittke
A new reference cigarette, the 3R4F, has been developed to replace the depleting supply of the 2R4F cigarette. The present study was designed to compare mainstream smoke chemistry and toxicity of the two reference cigarettes under the International Organization for Standardization (ISO) machine smoking conditions, and to further compare mainstream smoke chemistry and toxicological activity of the 3R4F cigarette by two different smoking regimens, i.e., the machine smoking conditions specified by ISO and the Health Canada intensive (HCI) smoking conditions.
The in vitro cytotoxicity and mutagenicity was determined in the neutral red uptake assay, the Salmonella reverse mutation assay, and the mouse lymphoma thymidine kinase assay. Additionally, a 90-day nose-only inhalation study in rats was conducted to assess the in vivo toxicity. The comparison of smoke chemistry between the two reference cigarettes found practically the same yields of total particulate matter (TPM), ‘tar’, nicotine, carbon monoxide, and most other smoke constituents. For both cigarettes, the in vitro cytotoxicity, mutagenicity, and in vivo toxicity showed the expected smoke-related effects compared to controls without smoke exposure. There were no meaningful differences between the 2R4F and 3R4F regarding these toxicological endpoints. The assessments for the 3R4F cigarette by smoking regimen found as a trivial effect, due to the higher amount of smoke generated per cigarette under HCI conditions, an increased yield of toxicant and higher toxicological activity per cigarette. However, per mg TPM, ‘tar’, or nicotine, the amounts of toxicants and the in vitro toxicity were generally lower under HCI conditions, but the in vivo activity was not different between the two machine smoking conditions. Overall, as the main result, the present study suggests equivalent smoke chemistry and in vitro and in vivo toxicity for the 2R4F and 3R4F reference cigarettes.