Masoud Rafiei, A.S. Gadgil, Hemlata Patil and Tongdi Jamir
The increases in air pollution over the metropolitan cities are a threat to human health and environment. An attempt has been made to evaluate the health consequences of indoor air pollution induced by Beam Rolling Mills Factory at Ahwaz (Iran). A questionnaire was prepared to obtain information on health of 481 workers, out of which 200 each were selected from exposed and non-exposed category by stratified randomized method. Fisher exact test and chi-square test were used to calculate the values. The study concludes that more than 80% of the workers have high exposure risk to diseases. Analysis of the health impacts reveals that exposed workers are more prone to various diseases as compared to the non-exposed workers. It is also observed that exposure to air pollutants might be the causative factor for various diseases among the smokers but also nonsmoking workers. The analysis also reveals that there is higher relative risk in occupational fatigue and cardio-vascular disease. Further, the study found that percentage of workers having various diseases is much higher in the indoor environment as compared to the outdoor environment
S. Fallahizadeh, R. Arjmand, A. Jelowdar, A. Rafiei and F. Kazemi
Echinococosis is a zoonotic disease caused by the larval stages of Echinococcus spp. that occurs in most parts of the world. Herein, we aimed to evaluate the genotypes of isolated hydatid cysts from slaughtered animals in Shush county, southwestern Iran. Totally, 96 hydatid cysts were collected, including 11 buffaloes, 13 cattle, 12 goat and 60 sheep. The PCR was done by a primer pair (BDI and 4s) to amplify ITS1 fragment. Four restriction endonucleases including AluI, HpaII, RsaI, and TaqI were used for RFLP products and enzymatic reactions were electrophoresed. Finally, twenty PCR products were sent for sequencing and phylogenetic tree was drawn with MEGA6. Molecular identification of 96 hydatid cysts demonstrated a distinctive 1000 bp fragment in all samples from four animal hosts. RFLP analysis showed similar digestion patterns in all samples. AluI digestion yielded 800 bp and 200 bp fragments, HpaII digestion made 700 bp and 300 bp fragments and RsaI digestion entailed 655 and 345segments. Moreover, TaqI rendered no digestion pattern on rDNA-ITS1 region. Additionally, E. granulosus sensu stricto (G1-3 complex) was the prevailing genotype in all livestock samples, according to PCR-RFLP and sequencing analyses.