Mass-produced printed circuit board (PCB) electrodes were used as electrochemical cells to detect the widely-used herbicide glyphosate. Square wave voltammetry (SWV) was used to determine the presence of glyphosate in aqueous Cu(NO3)2 solution. Optimal measurement conditions for the detection of glyphosate with PCB electrodes were found. It was determined that glyphosate was able to soak into the growing plants from the substrate. Glyphosate-contaminated plant juice was distinguished from control samples using the PCB electrode. Glyphosate-contaminated plants were found to have DNA mutations.